RAP1GAP Antibody - #DF4383

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Product: RAP1GAP Antibody
Catalog: DF4383
Description: Rabbit polyclonal antibody to RAP1GAP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Xenopus
Mol.Wt.: 73 KD; 73kD(Calculated).
Uniprot: P47736
RRID: AB_2836738

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RAP1GAP Antibody detects endogenous levels of total RAP1GAP.
RRID:
AB_2836738
Cite Format: Affinity Biosciences Cat# DF4383, RRID:AB_2836738.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0474; Rap1 GTPase activating protein 1; RAP1 GTPase activating protein; Rap1 GTPase-activating protein 1; RAP1GA1; Rap1ga1 protein; Rap1GAP; Rap1GAP1; RAP1GAPII; RAPGAP; RPGP1_HUMAN;

Immunogens

Immunogen:
Uniprot:

P47736(RPGP1_HUMAN) >>Visit HPA database.

Gene(ID):
RAP1GAP(5909)
Expression:
P47736 RPGP1_HUMAN:

Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.

Sequence:
MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDVIGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMENGSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Dog
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P47736 As Substrate

Site PTM Type Enzyme
Y27 Phosphorylation
S74 Phosphorylation
K232 Ubiquitination
K237 Ubiquitination
K262 Acetylation
K357 Ubiquitination
K378 Acetylation
K378 Ubiquitination
K384 Acetylation
S437 Phosphorylation
S439 Phosphorylation
S441 Phosphorylation
S460 Phosphorylation
S484 Phosphorylation P06493 (CDK1)
T486 Phosphorylation
S490 Phosphorylation P17612 (PRKACA)
S495 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation P17612 (PRKACA)
S515 Phosphorylation
T522 Phosphorylation
S525 Phosphorylation P53350 (PLK1)
S541 Phosphorylation
S542 Phosphorylation
T547 Phosphorylation
T548 Phosphorylation
S625 Phosphorylation

Research Backgrounds

Function:

GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.

Subcellular Location:

Golgi apparatus membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.

Subunit Structure:

Homodimer and heterodimer with RAP1B.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

References

1). Effect and mechanism of longkui yinxiao soup in treating psoriasis in mice. Frontiers in Pharmacology, 2023 (PubMed: 36992832) [IF=5.6]

Application: WB    Species: Mouse    Sample: skin tissues

FIGURE 8 Effects of LYS on the expression of Rap1 (Rap1 and Rap1gap) and MAPKs (p38, JNK, ERK1/2, and MEK3,6) signaling pathways in the psoriasis-like mouse model. Expression of p38, ERK, MEK3/6, Rap1gap, Rap1a, and Rap1b mRNAs in the back skin tissues was detected using reverse transcription–polymerase chain reaction (A) and the relative protein expression was detected using WB (B). The data are expressed as mean ± SD (n = 3).

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