RAPGEF3 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/5

Western blot analysis of extracts from Mouse testis, using RAPGEF3 Antibody at 1/1000 dilution.

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using RAPGEF3 Antibody at 1/1000 dilution.

DF4381 staining Hela cells by IF/ICC.

Fig.

Product:	RAPGEF3 Antibody
Catalog:	DF4381
Description:	Rabbit polyclonal antibody to RAPGEF3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	110/100 KD; 104kD(Calculated).
Uniprot:	O95398
RRID:	AB_2836736

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%)

Clonality:

Polyclonal

Specificity:

RAPGEF3 Antibody detects endogenous levels of total RAPGEF3.

RRID:

AB_2836736
Cite Format: Affinity Biosciences Cat# DF4381, RRID:AB_2836736.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; RAPGEF3;

Immunogens

Immunogen:

Uniprot:

O95398(RPGF3_HUMAN) >>Visit HPA database.

Gene(ID):

RAPGEF3(10411)

Expression:

O95398 RPGF3_HUMAN:

Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.

Sequence:

O95398 RPGF3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMVLRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGRQLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLLDEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLTVALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQGDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHFLRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGTPEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGSEQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICRPDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSLGLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIHQVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIKLAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHRVYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95398 As Substrate

O95398

Site	PTM Type	Source
S82	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
K268	Acetylation	Uniprot
T380	Phosphorylation	Uniprot
S494	Phosphorylation	Uniprot
S528	Phosphorylation	Uniprot
S864	Phosphorylation	Uniprot

Research Backgrounds

O95398_RPGF3_HUMAN

Function:

Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin.

Subcellular Location:

Endomembrane system.

Tissue Specificity:

Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney.

Subunit Structure:

Interacts with PDE3B.

Family&Domains:

The DEP domain is involved in membrane localization independent from regulation by cAMP.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Serotonergic synapse.

References

1). Epac1 signaling pathway mediates the damage and apoptosis of inner ear hair cells after noise exposure in a rat model. NEUROSCIENCE (PubMed: 33838290) [IF=3.3]

Application: WB Species: rat Sample: HEI-OC1 cells

Fig. 6. Expression of Epac1 in HEI-OC1 cells after oligomycin exposure. (A) Representative fluorescent microscopic images showing Epac1 expression in HEI-OC1 cells 12 h after oligomycin exposure; blue: DAPI; scale bar = 20 mm, n = 3. (B) Bar graph representing the intensity of Epac1 signal in HEI-OC1 cells. (C) Western blot analysis of Epac1 and Rap1 expression in HEI-OC1 cells 12 h after oligomycin exposure. b-actin was used as the loading control. The data represent the mean ± SEM, n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. OA.

Application: IF/ICC Species: rat Sample: HEI-OC1 cells

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

RAPGEF3 Antibody - #DF4381