Product: PMS2/PMS2CL Antibody
Catalog: DF4351
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human
Mol.Wt.: 21 kD; 21kD,96kD(Calculated).
Uniprot: Q68D20 | P54278
RRID: AB_2836719

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
PMS2/PMS2CL Antibody detects endogenous levels of total PMS2/PMS2CL.
RRID:
AB_2836719
Cite Format: Affinity Biosciences Cat# DF4351, RRID:AB_2836719.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PMS2 C terminal like protein; PMS2L; PMS2P13; DNA mismatch repair gene homologue; DNA mismatch repair protein PMS2; H_DJ0042M02.9; HNPCC4; Mismatch repair endonuclease PMS2; Mismatch repair gene PMSL2; MLH4; PMS 2; PMS1 homolog 2 mismatch repair system; PMS1 protein homolog 2; PMS2; PMS2 postmeiotic segregation increased 2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMS2_HUMAN; PMS2CL; PMSL2; Postmeiotic segregation increased, S. cerevisiae, 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MHAADLEKPMVEKQDQSPSLRTGEEKRDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMSSSSTSDAISDRGVLRPQKEAVSSSQGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSECVASTPGDRGSQEHVDSQEKAPETDDSFSDVDCHSNQEDTGCKFQVLPQPTNLTSPNTKVF

MERAESSSTEPAKAIKPIDRKSVHQICSGQVVLSLSTAVKELVENSLDAGATNIDLKLKDYGVDLIEVSDNGCGVEEENFEGLTLKHHTSKIQEFADLTQVETFGFRGEALSSLCALSDVTISTCHASAKVGTRLMFDHNGKIIQKTPYPRPRGTTVSVQQLFSTLPVRHKEFQRNIKKEYAKMVQVLHAYCIISAGIRVSCTNQLGQGKRQPVVCTGGSPSIKENIGSVFGQKQLQSLIPFVQLPPSDSVCEEYGLSCSDALHNLFYISGFISQCTHGVGRSSTDRQFFFINRRPCDPAKVCRLVNEVYHMYNRHQYPFVVLNISVDSECVDINVTPDKRQILLQEEKLLLAVLKTSLIGMFDSDVNKLNVSQQPLLDVEGNLIKMHAADLEKPMVEKQDQSPSLRTGEEKKDVSISRLREAFSLRHTTENKPHSPKTPEPRRSPLGQKRGMLSSSTSGAISDKGVLRPQKEAVSSSHGPSDPTDRAEVEKDSGHGSTSVDSEGFSIPDTGSHCSSEYAASSPGDRGSQEHVDSQEKAPKTDDSFSDVDCHSNQEDTGCKFRVLPQPTNLATPNTKRFKKEEILSSSDICQKLVNTQDMSASQVDVAVKINKKVVPLDFSMSSLAKRIKQLHHEAQQSEGEQNYRKFRAKICPGENQAAEDELRKEISKTMFAEMEIIGQFNLGFIITKLNEDIFIVDQHATDEKYNFEMLQQHTVLQGQRLIAPQTLNLTAVNEAVLIENLEIFRKNGFDFVIDENAPVTERAKLISLPTSKNWTFGPQDVDELIFMLSDSPGVMCRPSRVKQMFASRACRKSVMIGTALNTSEMKKLITHMGEMDHPWNCPHGRPTMRHIANLGVISQN

PTMs - Q68D20/P54278 As Substrate

Site PTM Type Enzyme
K13 Ubiquitination
K57 Sumoylation
K57 Ubiquitination
K142 Ubiquitination
K146 Ubiquitination
K210 Acetylation
S220 Phosphorylation
K224 Ubiquitination
K301 Ubiquitination
S373 Phosphorylation
K386 Ubiquitination
S403 Phosphorylation
S405 Phosphorylation
K413 Sumoylation
S425 Phosphorylation
S436 Phosphorylation
T439 Phosphorylation
S445 Phosphorylation
K450 Acetylation
S478 Phosphorylation
S482 Phosphorylation
T485 Phosphorylation
S523 Phosphorylation
T542 Phosphorylation
S547 Phosphorylation
T573 Phosphorylation
K581 Ubiquitination
S588 Phosphorylation
T597 Phosphorylation
S601 Phosphorylation
S603 Phosphorylation
S624 Phosphorylation
K630 Ubiquitination
S639 Phosphorylation
Y645 Phosphorylation
K647 Ubiquitination
K651 Sumoylation
K651 Ubiquitination
K748 Ubiquitination
K766 Ubiquitination
T777 Phosphorylation
S791 Phosphorylation
S793 Phosphorylation
S815 Phosphorylation
T824 Phosphorylation
S825 Phosphorylation
Site PTM Type Enzyme
S59 Phosphorylation
S92 Phosphorylation

Research Backgrounds

Family&Domains:

Belongs to the DNA mismatch repair MutL/HexB family.

Function:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of PMS2 and MLH1 (MutL alpha). Forms a ternary complex with MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3). Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with MTMR15/FAN1.

Family&Domains:

Belongs to the DNA mismatch repair MutL/HexB family.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

Restrictive clause

 

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