PPP1R3A Antibody - #DF4345

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Product: PPP1R3A Antibody
Catalog: DF4345
Description: Rabbit polyclonal antibody to PPP1R3A
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 140 KD; 126kD(Calculated).
Uniprot: Q16821
RRID: AB_2836713

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
PPP1R3A Antibody detects endogenous levels of total PPP1R3A.
RRID:
AB_2836713
Cite Format: Affinity Biosciences Cat# DF4345, RRID:AB_2836713.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Glycogen and sarcoplasmic reticulum binding subunit, skeletal muscle; Glycogen associated regulatory subunit of protein phosphatase 1; GM; PP1G; PPP1R3; PPP1R3A; PPR3A_HUMAN; Protein phosphatase 1 glycogen-associated regulatory subunit; Protein phosphatase 1 glycogen-targeting subunit, muscle; Protein phosphatase 1 regulatory subunit 3A; Protein phosphatase 1 regulatory subunit GM; protein phosphatase 1, regulatory (inhibitor) subunit 3A; Protein phosphatase 1, regulatory subunit 3; Protein phosphatase type-1 glycogen targeting subunit; RG1; Serine /threonine specific protein phosphatase; Type 1 protein phosphatase skeletal muscle glycogen targeting subunit;

Immunogens

Immunogen:
Uniprot:

Q16821(PPR3A_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R3A(5506)
Expression:
Q16821 PPR3A_HUMAN:

Skeletal muscle and heart.

Sequence:
MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSFGFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGSTSIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKIVLVPPYQKDGSKVEFCIRYETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLGDTSSDELVQLHTGSKEVLDDNANPAHGNGTVQIPCPSSDQLMAGNLNKKHEGGAKNIEVKDLGCLRRDFHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTEELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDLRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

PTMs - Q16821 As Substrate

Site PTM Type Enzyme
S46 Phosphorylation P51812 (RPS6KA3)
S48 Phosphorylation
S49 Phosphorylation
S65 Phosphorylation P51812 (RPS6KA3)
S332 Phosphorylation
S375 Phosphorylation
T735 Phosphorylation
T736 Phosphorylation
S739 Phosphorylation
T759 Phosphorylation
T822 Phosphorylation
S824 Phosphorylation
Y826 Phosphorylation
T939 Phosphorylation
T940 Phosphorylation
S1066 Phosphorylation
S1069 Phosphorylation

Research Backgrounds

Function:

Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Plays an important role in glycogen synthesis but is not essential for insulin activation of glycogen synthase (By similarity).

PTMs:

Phosphorylation at Ser-46 by ISPK stimulates the dephosphorylation of glycogen synthase and phosphorylase kinase.

Subcellular Location:

Membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Skeletal muscle and heart.

Subunit Structure:

Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen.

Family&Domains:

The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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