NDRG3 Antibody - #DF4340
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| Product: | NDRG3 Antibody |
| Catalog: | DF4340 |
| Description: | Rabbit polyclonal antibody to NDRG3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 45 KD; 41kD(Calculated). |
| Uniprot: | Q9UGV2 |
| RRID: | AB_2836708 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(80%), Horse(90%), Sheep(80%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
NDRG3 Antibody detects endogenous levels of total NDRG3.
RRID:
AB_2836708
Cite Format: Affinity Biosciences Cat# DF4340, RRID:AB_2836708.
Cite Format: Affinity Biosciences Cat# DF4340, RRID:AB_2836708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FLJ13556; N myc downstream regulated gene 3; N-myc downstream-regulated gene 3 protein; NDRG family member 3; NDRG3; NDRG3_HUMAN; Protein Ndr3; Protein NDRG3;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q9UGV2 NDRG3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDELQDVQLTEIKPLLNDKNGTRNFQDFDCQEHDIETTHGVVHVTIRGLPKGNRPVILTYHDIGLNHKSCFNAFFNFEDMQEITQHFAVCHVDAPGQQEGAPSFPTGYQYPTMDELAEMLPPVLTHLSLKSIIGIGVGAGAYILSRFALNHPELVEGLVLINVDPCAKGWIDWAASKLSGLTTNVVDIILAHHFGQEELQANLDLIQTYRMHIAQDINQDNLQLFLNSYNGRRDLEIERPILGQNDNKSKTLKCSTLLVVGDNSPAVEAVVECNSRLNPINTTLLKMADCGGLPQVVQPGKLTEAFKYFLQGMGYIPSASMTRLARSRTHSTSSSLGSGESPFSRSVTSNQSDGTQESCESPDVLDRHQTMEVSC
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
90
Dog
90
Rabbit
90
Bovine
80
Sheep
80
Xenopus
70
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UGV2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| Y308 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| T332 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S334 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| T348 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| C359 | S-Nitrosylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| T370 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| C375 | S-Nitrosylation | Uniprot |
Research Backgrounds
Tissue Specificity:
Ubiquitous. Highly expressed in brain.
Family&Domains:
Belongs to the NDRG family.
References
1). Hypoxic postconditioning attenuates apoptosis via inactivation of adenosine A2a receptor through NDRG3-Raf-ERK pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017
(PubMed: 28743501)
[IF=3.1]
Application: WB Species: human Sample:
Fig.2 Protein Expression of NDRD3-Raf-ERK pathway, A2a receptors, Cytochrome C.
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