DDX20 Antibody - #DF4332

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Product: DDX20 Antibody
Catalog: DF4332
Description: Rabbit polyclonal antibody to DDX20
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 92 KD; 92kD(Calculated).
Uniprot: Q9UHI6
RRID: AB_2836700

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(100%), Horse(89%), Sheep(100%), Rabbit(89%), Dog(89%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DDX20 Antibody detects endogenous levels of total DDX20.
RRID:
AB_2836700
Cite Format: Affinity Biosciences Cat# DF4332, RRID:AB_2836700.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Component of gems 3; DDX 20; ddx20; DDX20_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 20; DEAD box protein 20; DEAD box protein DP 103; DEAD box protein DP103; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20; DEAD/H BOX 20; DKFZP434H052; DP 103; DP103; Gemin-3; Gemin3; Probable ATP dependent RNA helicase DDX20; Probable ATP-dependent RNA helicase ddx20; SMN interacting protein;

Immunogens

Immunogen:
Uniprot:

Q9UHI6(DDX20_HUMAN) >>Visit HPA database.

Gene(ID):
DDX20(11218)
Expression:
Q9UHI6 DDX20_HUMAN:

Ubiquitous.

Sequence:
MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAEPADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIALDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKKCHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQMLAVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHLQELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHCRVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEENMMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIERTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDRQSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEHYIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVIEQRVPVLASSSQSGDSESDSDSYSSRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQMKTRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAYYRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Chicken
100
Pig
89
Horse
89
Dog
89
Rabbit
89
Xenopus
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHI6 As Substrate

Site PTM Type Enzyme
S48 Phosphorylation
S86 Phosphorylation
K91 Ubiquitination
K107 Ubiquitination
K175 Ubiquitination
K180 Ubiquitination
S187 Phosphorylation
K192 Ubiquitination
Y199 Phosphorylation
S268 Phosphorylation
S269 Phosphorylation
S272 Phosphorylation
K277 Ubiquitination
K281 Ubiquitination
K296 Ubiquitination
S320 Phosphorylation
K332 Ubiquitination
K354 Ubiquitination
S442 Phosphorylation
T461 Phosphorylation
Y462 Phosphorylation
K473 Ubiquitination
K478 Ubiquitination
K487 Methylation
K487 Ubiquitination
S495 Phosphorylation
S500 Phosphorylation
S505 Phosphorylation
K507 Ubiquitination
K519 Ubiquitination
S520 Phosphorylation
S522 Phosphorylation
K529 Ubiquitination
T531 Phosphorylation
S532 Phosphorylation
K534 Ubiquitination
S542 Phosphorylation
T552 Phosphorylation
S560 Phosphorylation
K565 Ubiquitination
K608 Ubiquitination
Y616 Phosphorylation
K634 Ubiquitination
S649 Phosphorylation
S652 Phosphorylation
S654 Phosphorylation
S656 Phosphorylation
K671 Ubiquitination
S672 Phosphorylation
Y673 Phosphorylation
S677 Phosphorylation
S678 Phosphorylation
S685 Phosphorylation
T688 Phosphorylation
R693 Methylation
S695 Phosphorylation
S703 Phosphorylation
T705 Phosphorylation
K710 Ubiquitination
Y711 Phosphorylation
S714 Phosphorylation
K720 Methylation
K720 Ubiquitination
S742 Phosphorylation
S743 Phosphorylation
T748 Phosphorylation
Y756 Phosphorylation
S765 Phosphorylation
Y770 Phosphorylation

Research Backgrounds

Function:

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).

Subcellular Location:

Cytoplasm. Nucleus>Gem.
Note: Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies (Gems).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Part of the core SMN complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Part of the SMN-Sm complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8, STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG. Interacts directly with GEMIN5. Interacts directly with SNUPN. Interacts with PPP4R2. Interacts with FOXL2. Interacts with EBV EBNA2 and EBNA3C. Interacts with NANOS1 and PUM2.

Family&Domains:

Belongs to the DEAD box helicase family. DDX20 subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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