SLU7 Antibody - #DF4330

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Product: SLU7 Antibody
Catalog: DF4330
Description: Rabbit polyclonal antibody to SLU7
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 65 KD; 68kD(Calculated).
Uniprot: O95391
RRID: AB_2836698

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SLU7 Antibody detects endogenous levels of total SLU7.
RRID:
AB_2836698
Cite Format: Affinity Biosciences Cat# DF4330, RRID:AB_2836698.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

9G8; hSlu7; MGC9280; Pre mRNA splicing factor SLU7; Pre-mRNA-splicing factor slu7; SLU 7; slu7; SLU7 splicing factor homolog (S. cerevisiae); SLU7 splicing factor homolog; SLU7_HUMAN; splicing factor; Step II splicing factor SLU7; Zinc knuckle motif containing;

Immunogens

Immunogen:
Uniprot:

O95391(SLU7_HUMAN) >>Visit HPA database.

Gene(ID):
SLU7(10569)
Sequence:
MSATVVDAVNAAPLSGSKEMSLEEPKKMTREDWRKKKELEEQRKLGNAPAEVDEEGKDINPHIPQYISSVPWYIDPSKRPTLKHQRPQPEKQKQFSSSGEWYKRGVKENSIITKYRKGACENCGAMTHKKKDCFERPRRVGAKFTGTNIAPDEHVQPQLMFDYDGKRDRWNGYNPEEHMKIVEEYAKVDLAKRTLKAQKLQEELASGKLVEQANSPKHQWGEEEPNSQMEKDHNSEDEDEDKYADDIDMPGQNFDSKRRITVRNLRIREDIAKYLRNLDPNSAYYDPKTRAMRENPYANAGKNPDEVSYAGDNFVRYTGDTISMAQTQLFAWEAYDKGSEVHLQADPTKLELLYKSFKVKKEDFKEQQKESILEKYGGQEHLDAPPAELLLAQTEDYVEYSRHGTVIKGQERAVACSKYEEDVKIHNHTHIWGSYWKEGRWGYKCCHSFFKYSYCTGEAGKEIVNSEECIINEITGEESVKKPQTLMELHQEKLKEEKKKKKKKKKKHRKSSSDSDDEEKKHEKLKKALNAEEARLLHVKETMQIDERKRPYNSMYETREPTEEEMEAYRMKRQRPDDPMASFLGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95391 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S15 Phosphorylation
K44 Sumoylation
K78 Ubiquitination
K83 Ubiquitination
K93 Ubiquitination
Y102 Phosphorylation
K103 Ubiquitination
K107 Ubiquitination
S110 Phosphorylation
K131 Ubiquitination
Y163 Phosphorylation
K166 Ubiquitination
K187 Sumoylation
K187 Ubiquitination
K199 Sumoylation
K199 Ubiquitination
K208 Ubiquitination
S215 Phosphorylation
S227 Phosphorylation
S235 Phosphorylation
Y243 Phosphorylation
S256 Phosphorylation
K257 Sumoylation
K257 Ubiquitination
T261 Phosphorylation
K273 Ubiquitination
Y284 Phosphorylation
K288 Ubiquitination
Y297 Phosphorylation
K302 Acetylation
K302 Ubiquitination
S308 Phosphorylation
K349 Ubiquitination
Y354 Phosphorylation
K355 Ubiquitination
K369 Ubiquitination
K375 Ubiquitination
Y397 Phosphorylation
Y400 Phosphorylation
K408 Ubiquitination
K418 Sumoylation
S466 Phosphorylation
K482 Sumoylation
K482 Ubiquitination
K493 Ubiquitination
S513 Phosphorylation
S515 Phosphorylation
K540 Acetylation
K540 Ubiquitination
S554 Phosphorylation
S582 Phosphorylation

Research Backgrounds

Function:

Required for pre-mRNA splicing as component of the spliceosome. Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.
Note: Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing. Translocates from the nucleus to the cytoplasm after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of pre-catalytic, catalytic and post-catalytic spliceosomes. Associates with the spliceosome prior to recognition of the 3'-splice site for step II, probably during catalysis of step I.

Family&Domains:

The CCHC-type zinc finger is required to retain the protein within the nucleus and prevent its shuttle back to the cytoplasm via the CRM1 pathway.

Belongs to the SLU7 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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