CDC40 Antibody | Affinity Biosciences

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Western blot analysis of extracts from VERO, using CDC40 Antibody.

Product:	CDC40 Antibody
Catalog:	DF4326
Description:	Rabbit polyclonal antibody to CDC40
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	66 KD; 66kD(Calculated).
Uniprot:	O60508
RRID:	AB_2836694

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

CDC40 Antibody detects endogenous levels of total CDC40.

RRID:

AB_2836694
Cite Format: Affinity Biosciences Cat# DF4326, RRID:AB_2836694.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cdc40; Cell division cycle 40; Cell division cycle 40 homolog; EH binding protein 3; EH-binding protein 3; Ehb3; hPRP17; Pre mRNA processing factor 17; Pre mRNA splicing factor 17; Pre-mRNA-processing factor 17; PRP17; PRP17 homolog; PRP17_HUMAN; PRPF17;

Immunogens

Immunogen:

Uniprot:

O60508(PRP17_HUMAN) >>Visit HPA database.

Gene(ID):

CDC40(51362)

Sequence:

O60508 PRP17_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALDPSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSEEEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWDGLIKLWD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60508 As Substrate

O60508

Site	PTM Type	Source
S2	Phosphorylation	Uniprot
Y12	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
S16	Phosphorylation	Uniprot
S18	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
S22	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
S27	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
S46	Phosphorylation	Uniprot
S49	Phosphorylation	Uniprot
S56	Phosphorylation	Uniprot
Y81	Phosphorylation	Uniprot
T84	Phosphorylation	Uniprot
Y85	Phosphorylation	Uniprot
Y114	Phosphorylation	Uniprot
Y135	Phosphorylation	Uniprot
Y137	Phosphorylation	Uniprot
K198	Ubiquitination	Uniprot
K226	Acetylation	Uniprot
K228	Acetylation	Uniprot
K380	Ubiquitination	Uniprot
K387	Ubiquitination	Uniprot
K399	Acetylation	Uniprot
S435	Phosphorylation	Uniprot

Research Backgrounds

O60508_PRP17_HUMAN

Function:

Required for pre-mRNA splicing as component of the activated spliceosome.

Subcellular Location:

Nucleus. Nucleus speckle.

Subunit Structure:

Component of the pre-catalytic and catalytic spliceosome complexes. Component of the postcatalytic spliceosome P complex.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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CDC40 Antibody - #DF4326