SF3B14 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat kidney, using SF3B14 Antibody.

DF4325 at 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P.

DF4325 at 1/100 staining Human ovarian cancer by IHC-P.

Product:	SF3B14 Antibody
Catalog:	DF4325
Description:	Rabbit polyclonal antibody to SF3B14
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	20 KD; 15kD(Calculated).
Uniprot:	Q9Y3B4
RRID:	AB_2836693

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SF3B14 Antibody detects endogenous levels of total SF3B14.

RRID:

AB_2836693
Cite Format: Affinity Biosciences Cat# DF4325, RRID:AB_2836693.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Ht006; P14; PM14_HUMAN; Pre mRNA branch site protein p14; Pre-mRNA branch site protein p14; SAP14; SF3B 14 kDa subunit; SF3B14; SF3B14a; Splicing factor 3B, 14 kDa subunit;

Immunogens

Immunogen:

Uniprot:

Q9Y3B4(SF3B6_HUMAN) >>Visit HPA database.

Gene(ID):

SF3B6(51639)

Sequence:

Q9Y3B4 SF3B6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3B4 As Substrate

Q9Y3B4

Site	PTM Type	Source
K7	Sumoylation	Uniprot
K7	Ubiquitination	Uniprot
K29	Acetylation	Uniprot
K29	Ubiquitination	Uniprot
Y36	Phosphorylation	Uniprot
K41	Acetylation	Uniprot
K41	Ubiquitination	Uniprot
Y61	Phosphorylation	Uniprot
Y64	Phosphorylation	Uniprot
K71	Ubiquitination	Uniprot
C74	S-Nitrosylation	Uniprot
C83	S-Nitrosylation	Uniprot
Y86	Phosphorylation	Uniprot
K100	Ubiquitination	Uniprot
K111	Ubiquitination	Uniprot
K116	Acetylation	Uniprot
K116	Ubiquitination	Uniprot

Research Backgrounds

Q9Y3B4_SF3B6_HUMAN

Function:

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. Within the SF3B complex interacts directly with SF3B1 Ref.20). The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.

Family&Domains:

Belongs to the SF3B6 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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SF3B14 Antibody - #DF4325