Product: KCNA5 Antibody
Catalog: DF4313
Description: Rabbit polyclonal antibody to KCNA5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 68 KD; 67kD(Calculated).
Uniprot: P22460
RRID: AB_2836681

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KCNA5 Antibody detects endogenous levels of total KCNA5.
RRID:
AB_2836681
Cite Format: Affinity Biosciences Cat# DF4313, RRID:AB_2836681.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATFB7; cardiac potassium channel; HCK1; HK2; HPCN1; insulinoma and islet potassium channel; KCNA5; KCNA5_HUMAN; KV1.5; PCN1; potassium channel 1; Potassium channel, insulinoma and islet cell; Potassium channel, voltage-gated, shaker-related subfamily, member 5; Potassium voltage gated channel subfamily A member 5; Potassium voltage-gated channel subfamily A member 5; Voltage-gated potassium channel HK2; voltage-gated potassium channel protein Kv1.5; Voltage-gated potassium channel subunit Kv1.5;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P22460 KCNA5_HUMAN:

Pancreatic islets and insulinoma.

Sequence:
MEIALVPLENGGAMTVRGGDEARAGCGQATGGELQCPPTAGLSDGPKEPAPKGRGAQRDADSGVRPLPPLPDPGVRPLPPLPEELPRPRRPPPEDEEEEGDPGLGTVEDQALGTASLHHQRVHINISGLRFETQLGTLAQFPNTLLGDPAKRLRYFDPLRNEYFFDRNRPSFDGILYYYQSGGRLRRPVNVSLDVFADEIRFYQLGDEAMERFREDEGFIKEEEKPLPRNEFQRQVWLIFEYPESSGSARAIAIVSVLVILISIITFCLETLPEFRDERELLRHPPAPHQPPAPAPGANGSGVMAPPSGPTVAPLLPRTLADPFFIVETTCVIWFTFELLVRFFACPSKAGFSRNIMNIIDVVAIFPYFITLGTELAEQQPGGGGGGQNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGKTLQASMRELGLLIFFLFIGVILFSSAVYFAEADNQGTHFSSIPDAFWWAVVTMTTVGYGDMRPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETDHEEPAVLKEEQGTQSQGPGLDRGVQRKVSGSRGSFCKAGGTLENADSARRGSCPLEKCNVKAKSNVDLRRSLYALCLDTSRETDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
90
Xenopus
60
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22460 As Substrate

Site PTM Type Enzyme
T15 Phosphorylation P17252 (PRKCA)
S62 Phosphorylation
S192 Phosphorylation
Y203 Phosphorylation
K221 Sumoylation
C331 S-Nitrosylation
C346 S-Nitrosylation
S414 Phosphorylation
S498 Phosphorylation
K536 Sumoylation
S557 Phosphorylation
S559 Phosphorylation
S575 Phosphorylation
S592 Phosphorylation

Research Backgrounds

Function:

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. Homotetrameric channels display rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets. Isoform 2 exhibits a voltage-dependent recovery from inactivation and an excessive cumulative inactivation.

PTMs:

Sumoylated on Lys-221, and Lys-536, preferentially with SUMO3. Sumoylation regulates the voltage sensitivity of the channel.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Pancreatic islets and insulinoma.

Subunit Structure:

Homotetramer and heterotetramer of potassium channel proteins. Interacts with DLG1, which enhances channel currents. Forms a ternary complex with DLG1 and CAV3 (By similarity). Interacts with KCNAB1. Interacts with UBE2I.

Family&Domains:

The amino terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.5/KCNA5 sub-subfamily.

References

1). Role of Ion Channels in Chronic Intermittent Hypoxia-Induced Atrial Remodeling in Rats. LIFE SCIENCES, 2020 (PubMed: 32417371) [IF=6.1]

Application: WB    Species: rat    Sample: atrial

Fig. 6.| Protein levels of Nav1.5, Cav1.2, Kv4.2, Kv4.3, Kv1.5, NCX1, RyR2, p-RyR2, CaMKII, p-CaMKII confirmed by Western Blotting in two groups (n = 10). And p-RyR2/RyR2, p-CaMKII/CaMKII were also calculated and compared between two groups. LA -left atrial, RA -right atrial.

2). Effects of different intensity endurance training on atrial fibrillation in Rats. Research Square, 2024

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