RDX Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from HuvEc cells, using RDX antibody.

Product:	RDX Antibody
Catalog:	DF4376
Description:	Rabbit polyclonal antibody to RDX
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	69 KD; 69kD(Calculated).
Uniprot:	P35241
RRID:	AB_2836664

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

RDX Antibody detects endogenous levels of total RDX.

RRID:

AB_2836664
Cite Format: Affinity Biosciences Cat# DF4376, RRID:AB_2836664.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CB567; CG12537; DFNB24; ESP10; Hh-induced MATH and BTB domain-containing protein; HIB; Moesin-B; Protein roadkill; RADI_HUMAN; Radixin; RDX;

Immunogens

Immunogen:

Uniprot:

P35241(RADI_HUMAN) >>Visit HPA database.

Gene(ID):

RDX(5962)

Sequence:

P35241 RADI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTVGLREVWFFGLQYVDSKGYSTWLKLNKKVTQQDVKKENPLQFKFRAKFFPEDVSEELIQEITQRLFFLQVKEAILNDEIYCPPETAVLLASYAVQAKYGDYNKEIHKPGYLANDRLLPQRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVNYFEIKNKKGTELWLGVDALGLNIYEHDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERAQLENEKKKREIAEKEKERIEREKEELMERLKQIEEQTIKAQKELEEQTRKALELDQERKRAKEEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQEDLEKTKEELKTVMSAPPPPPPPPVIPPTENEHDEHDENNAEASAELSNEGVMNHRSEEERVTETQKNERVKKQLQALSSELAQARDETKKTQNDVLHAENVKAGRDKYKTLRQIRQGNTKQRIDEFEAM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Bovine

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35241 As Substrate

P35241

Site	PTM Type	Enzyme	Source
K3	Ubiquitination		Uniprot
K35	Ubiquitination		Uniprot
Y55	Phosphorylation		Uniprot
T66	Phosphorylation		Uniprot
K72	Ubiquitination		Uniprot
K79	Acetylation		Uniprot
K79	Ubiquitination		Uniprot
K83	Ubiquitination		Uniprot
Y128	Phosphorylation		Uniprot
Y134	Phosphorylation		Uniprot
Y137	Phosphorylation		Uniprot
K139	Ubiquitination		Uniprot
K143	Ubiquitination		Uniprot
R151	Methylation		Uniprot
K162	Ubiquitination		Uniprot
K165	Ubiquitination		Uniprot
K209	Ubiquitination		Uniprot
K233	Ubiquitination		Uniprot
K237	Ubiquitination		Uniprot
S243	Phosphorylation		Uniprot
S249	Phosphorylation		Uniprot
K253	Acetylation		Uniprot
K253	Ubiquitination		Uniprot
K254	Ubiquitination		Uniprot
K258	Acetylation		Uniprot
K258	Ubiquitination		Uniprot
K262	Acetylation		Uniprot
K262	Sumoylation		Uniprot
K262	Ubiquitination		Uniprot
K263	Acetylation		Uniprot
K263	Ubiquitination		Uniprot
Y270	Phosphorylation		Uniprot
R273	Methylation		Uniprot
Y291	Phosphorylation		Uniprot
K296	Ubiquitination		Uniprot
T299	Phosphorylation		Uniprot
K306	Methylation		Uniprot
K306	Ubiquitination		Uniprot
K335	Ubiquitination		Uniprot
K344	Acetylation		Uniprot
K344	Ubiquitination		Uniprot
K352	Ubiquitination		Uniprot
K360	Ubiquitination		Uniprot
T369	Phosphorylation		Uniprot
K400	Ubiquitination		Uniprot
K405	Ubiquitination		Uniprot
K427	Ubiquitination		Uniprot
K435	Ubiquitination		Uniprot
K448	Ubiquitination		Uniprot
K526	Ubiquitination		Uniprot
S533	Phosphorylation		Uniprot
T542	Phosphorylation		Uniprot
K544	Ubiquitination		Uniprot
T545	Phosphorylation		Uniprot
K556	Acetylation		Uniprot
K556	Ubiquitination		Uniprot
Y562	Phosphorylation		Uniprot
T564	Phosphorylation	O94804 (STK10) , P41279 (MAP3K8) , Q04759 (PRKCQ) , P25098 (GRK2) , O95819 (MAP4K4) , O75116 (ROCK2) , Q5S007 (LRRK2) , Q13464 (ROCK1)	Uniprot
T573	Phosphorylation		Uniprot

Research Backgrounds

P35241_RADI_HUMAN

Function:

Probably plays a crucial role in the binding of the barbed end of actin filaments to the plasma membrane.

PTMs:

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity).

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton. Cleavage furrow. Cell projection>Microvillus.
Note: Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively.

Subunit Structure:

Binds SLC9A3R1. Interacts with NHERF1, NHERF2, LAYN, MME/NEP and ICAM2. Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain). Interacts with SPN and CD44 (By similarity).

Family&Domains:

The N-terminal domain interacts with the C-terminal domain of LAYN. An interdomain interaction between its N-terminal and C-terminal domains inhibits its ability to bind LAYN. In the presence of acidic phospholipids, the interdomain interaction is inhibited and this enhances binding to LAYN (By similarity).

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

Restrictive clause

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For Research Use Only.
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RDX Antibody - #DF4376