PVRL4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from NIH/3T3 cells, using PVRL4 antibody.

DF4302 at 1/100 staining human lung cancer by IHC-P.

Product:	PVRL4 Antibody
Catalog:	DF4302
Description:	Rabbit polyclonal antibody to PVRL4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	55 KD; 55kD(Calculated).
Uniprot:	Q96NY8
RRID:	AB_2836653

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

PVRL4 Antibody detects endogenous levels of total PVRL4.

RRID:

AB_2836653
Cite Format: Affinity Biosciences Cat# DF4302, RRID:AB_2836653.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp686K05193; EDSS1; Ig superfamily receptor LNIR; Nectin 4; Nectin-4; poliovirus receptor-related 4; Processed poliovirus receptor-related protein 4; PRR4; pvrl4; PVRL4_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q96NY8(NECT4_HUMAN) >>Visit HPA database.

Gene(ID):

NECTIN4(81607)

Expression:

Q96NY8 NECT4_HUMAN:

Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.

Sequence:

Q96NY8 NECT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLSLGAEMWGPEAWLLLLLLLASFTGRCPAGELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASVVVVGVIAALLFCLLVVVVVLMSRYHRRKAQQMTQKYEEELTLTRENSIRRLHSHHTDPRSQPEESVGLRAEGHPDSLKDNSSCSVMSEEPEGRSYSTLTTVREIETQTELLSPGSGRAEEEEDQDEGIKQAMNHFVQENGTLRAKPTGNGIYINGRGHLV

PTMs - Q96NY8 As Substrate

Q96NY8

Site	PTM Type	Source
S58	Phosphorylation	Uniprot
T391	Phosphorylation	Uniprot
T393	Phosphorylation	Uniprot
S397	Phosphorylation	Uniprot
S426	Phosphorylation	Uniprot
S444	Phosphorylation	Uniprot
Y445	Phosphorylation	Uniprot
S446	Phosphorylation	Uniprot
T447	Phosphorylation	Uniprot
S462	Phosphorylation	Uniprot
T491	Phosphorylation	Uniprot
Y502	Phosphorylation	Uniprot

Research Backgrounds

Q96NY8_NECT4_HUMAN

Function:

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.

(Microbial infection) Acts as a receptor for measles virus.

PTMs:

The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell junction>Adherens junction.
Note: Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).

Secreted.
Note: The secreted form is found in breast tumor patients (PubMed:15784625).

Tissue Specificity:

Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.

Subunit Structure:

Self-associates. Interacts via its Ig-like V-type domain with NECTIN1 Ig-like V-type domain. Interacts via its C-terminus with AFDN.

(Microbial infection) Interacts with measles virus Hemagglutinin protein.

Family&Domains:

Belongs to the nectin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

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PVRL4 Antibody - #DF4302