SERPING1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HuvEc cells, using SERPING1 antibody.

Product:	SERPING1 Antibody
Catalog:	DF4299
Description:	Rabbit polyclonal antibody to SERPING1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig
Mol.Wt.:	55 KD; 55kD(Calculated).
Uniprot:	P05155
RRID:	AB_2836650

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(91%)

Clonality:

Polyclonal

Specificity:

SERPING1 Antibody detects endogenous levels of total SERPING1.

RRID:

AB_2836650
Cite Format: Affinity Biosciences Cat# DF4299, RRID:AB_2836650.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C1 esterase inhibitor; C1 Inh; C1 inhibiting factor; C1 inhibitor; C1-inhibiting factor; C1IN; C1Inh; C1NH; complement component 1 inhibitor; esterase inhibitor; HAE1; HAE2; IC1_HUMAN; Plasma protease C1 inhibitor; Serine (or cysteine) proteinase inhibitor clade G member 1; serine/cysteine proteinase inhibitor clade G member 1; Serpin G1; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1; SERPING1;

Immunogens

Immunogen:

Uniprot:

P05155(IC1_HUMAN) >>Visit HPA database.

Gene(ID):

SERPING1(710)

Sequence:

P05155 IC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASRLTLLTLLLLLLAGDRASSNPNATSSSSQDPESLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNSTTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05155 As Substrate

P05155

Site	PTM Type	Source
R4	Methylation	Uniprot
N25	N-Glycosylation	Uniprot
T47	O-Glycosylation	Uniprot
T48	O-Glycosylation	Uniprot
S64	O-Glycosylation	Uniprot
N69	N-Glycosylation	Uniprot
T71	O-Glycosylation	Uniprot
N81	N-Glycosylation	Uniprot
T83	O-Glycosylation	Uniprot
T88	O-Glycosylation	Uniprot
T92	O-Glycosylation	Uniprot
T96	O-Glycosylation	Uniprot
N238	N-Glycosylation	Uniprot
N253	N-Glycosylation	Uniprot
Y294	Phosphorylation	Uniprot
S296	Phosphorylation	Uniprot
K316	Acetylation	Uniprot
K321	Acetylation	Uniprot
N352	N-Glycosylation	Uniprot
S375	Phosphorylation	Uniprot
S460	Phosphorylation	Uniprot

Research Backgrounds

P05155_IC1_HUMAN

Function:

Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

PTMs:

Highly glycosylated (49%) with N- and O-glycosylation. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan heterogeneity at Asn-25: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).

Can be proteolytically cleaved by E.coli stcE.

Subcellular Location:

Secreted.

Subunit Structure:

Binds to E.coli stcE which allows localization of SERPING1 to cell membranes thus protecting the bacteria against complement-mediated lysis. Interacts with MASP1.

Family&Domains:

Belongs to the serpin family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

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For Research Use Only.
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SERPING1 Antibody - #DF4299