PKP2 Antibody - #DF4297

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Product: PKP2 Antibody
Catalog: DF4297
Description: Rabbit polyclonal antibody to PKP2
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 97 KD; 97kD(Calculated).
Uniprot: Q99959
RRID: AB_2836648

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(83%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PKP2 Antibody detects endogenous levels of total PKP2.
RRID:
AB_2836648
Cite Format: Affinity Biosciences Cat# DF4297, RRID:AB_2836648.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARVD 9; ARVD9; PKP 2; PKP2; PKP2_HUMAN; Plakophilin-2; Plakophilin2;

Immunogens

Immunogen:
Uniprot:

Q99959(PKP2_HUMAN) >>Visit HPA database.

Gene(ID):
PKP2(5318)
Expression:
Q99959 PKP2_HUMAN:

Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata.

Sequence:
MAAPGAPAEYGYIRTVLGQQILGQLDSSSLALPSEAKLKLAGSSGRGGQTVKSLRIQEQVQQTLARKGRSSVGNGNLHRTSSVPEYVYNLHLVENDFVGGRSPVPKTYDMLKAGTTATYEGRWGRGTAQYSSQKSVEERSLRHPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPPRYARSEIVGVSRAGTTSRQRHFDTYHRQYQHGSVSDTVFDSIPANPALLTYPRPGTSRSMGNLLEKENYLTAGLTVGQVRPLVPLQPVTQNRASRSSWHQSSFHSTRTLREAGPSVAVDSSGRRAHLTVGQAAAGGSGNLLTERSTFTDSQLGNADMEMTLERAVSMLEADHMLPSRISAAATFIQHECFQKSEARKRVNQLRGILKLLQLLKVQNEDVQRAVCGALRNLVFEDNDNKLEVAELNGVPRLLQVLKQTRDLETKKQITDHTVNLRSRNGWPGAVAHACNPSTLGGQGGRITRSGVRDQPDQHGLLWNLSSNDKLKNLMITEALLTLTENIIIPFSGWPEGDYPKANGLLDFDIFYNVTGCLRNMSSAGADGRKAMRRCDGLIDSLVHYVRGTIADYQPDDKATENCVCILHNLSYQLEAELPEKYSQNIYIQNRNIQTDNNKSIGCFGSRSRKVKEQYQDVPMPEEKSNPKGVEWLWHSIVIRMYLSLIAKSVRNYTQEASLGALQNLTAGSGPMPTSVAQTVVQKESGLQHTRKMLHVGDPSVKKTAISLLRNLSRNLSLQNEIAKETLPDLVSIIPDTVPSTDLLIETTASACYTLNNIIQNSYQNARDLLNTGGIQKIMAISAGDAYASNKASKAASVLLYSLWAHTELHHAYKKAQFKKTDFVNSRTAKAYHSLKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Pig
92
Horse
92
Bovine
92
Sheep
83
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99959 As Substrate

Site PTM Type Enzyme
Y10 Phosphorylation
Y12 Phosphorylation
K37 Ubiquitination
K39 Methylation
K39 Ubiquitination
S43 Phosphorylation
S44 Phosphorylation
R46 Methylation
K52 Methylation
K52 Ubiquitination
S53 Phosphorylation
T63 Phosphorylation
S71 Phosphorylation
T80 Phosphorylation
S81 Phosphorylation
S82 Phosphorylation
Y86 Phosphorylation
Y88 Phosphorylation
S102 Phosphorylation
K106 Ubiquitination
T107 Phosphorylation
Y108 Phosphorylation
K112 Methylation
K112 Ubiquitination
T118 Phosphorylation
Y119 Phosphorylation
R125 Methylation
T127 Phosphorylation
Y130 Phosphorylation
S131 Phosphorylation
S132 Phosphorylation
K134 Ubiquitination
S135 Phosphorylation
S140 Phosphorylation
S151 Phosphorylation
S154 Phosphorylation
S155 Phosphorylation
Y161 Phosphorylation
T162 Phosphorylation
S164 Phosphorylation
Y166 Phosphorylation
Y168 Phosphorylation
S169 Phosphorylation
S172 Phosphorylation
T177 Phosphorylation
S183 Phosphorylation
Y194 Phosphorylation
S197 Phosphorylation
S203 Phosphorylation
R204 Methylation
T207 Phosphorylation
T208 Phosphorylation
T216 Phosphorylation
Y217 Phosphorylation
Y221 Phosphorylation
S225 Phosphorylation
S233 Phosphorylation
T242 Phosphorylation
Y243 Phosphorylation
T248 Phosphorylation
S251 Phosphorylation
K258 Ubiquitination
Y261 Phosphorylation
T263 Phosphorylation
T267 Phosphorylation
S288 Phosphorylation
S289 Phosphorylation
S293 Phosphorylation
S294 Phosphorylation
S297 Phosphorylation
R302 Methylation
S312 Phosphorylation
S329 Phosphorylation
S342 Phosphorylation
S358 Phosphorylation
R390 Methylation
R395 Methylation
K405 Ubiquitination
K430 Ubiquitination
K447 Ubiquitination
T459 Phosphorylation
T462 Phosphorylation
Y597 Phosphorylation
Y616 Phosphorylation
Y626 Phosphorylation
Y631 Phosphorylation
K643 Ubiquitination
S650 Phosphorylation
K656 Ubiquitination
Y659 Phosphorylation
Y686 Phosphorylation
S693 Phosphorylation
K736 Ubiquitination
S744 Phosphorylation
K747 Ubiquitination
S761 Phosphorylation
Y831 Phosphorylation
S833 Phosphorylation
K864 Ubiquitination
S870 Phosphorylation
K874 Ubiquitination
Y876 Phosphorylation
S878 Phosphorylation

Research Backgrounds

Function:

May play a role in junctional plaques.

Subcellular Location:

Nucleus. Cell junction>Desmosome.
Note: Nuclear and associated with desmosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in heart right ventricle (at protein level). Widely expressed. Found at desmosomal plaques in simple and stratified epithelia and in non-epithelial tissues such as myocardium and lymph node follicles. In most stratified epithelia found in the desmosomes of the basal cell layer and seems to be absent from suprabasal strata.

Subunit Structure:

Interacts with DSC2. Interacts with JUP and desmoplakin/DSP.

Family&Domains:

Belongs to the beta-catenin family.

Research Fields

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

References

1). Identification of PKP 2/3 as potential biomarkers of ovarian cancer based on bioinformatics and experiments. Cancer Cell International, 2020 (PubMed: 33088217) [IF=5.8]

Application: IHC    Species: Human    Sample: ovarian tissue

Fig. 2 The expression and prognostic values of PKP1/2/3 protein in ovarian cancer (Human Protein Atlas and Kaplan–Meier plotter). A The expression of PKP1 (a), PKP2 (b) and PKP3 (c) in OC and normal tissues stained by immunohistochemistry. B (a–c) Relationship between the expression of PKP1 (a), PKP2(b), PKP3(c) and OS in patients with OC; (d–f) Relationship between the expression of PKP1 (d), PKP2 (e), PKP3( f) and PFS in patients with OC; (g–i) Relationship between the expression of PKP1 (g), PKP2 (h), PKP3 (i) and OS in OC patients with TP53 mutation; (j–l) Relationship between the expression of PKP1 (j), PKP2 (k), PKP3 (l) and PFS in OC patients with TP53 mutation. PKP plakophilin, OC ovarian cancer, OS Overall Survival, PFS progression-free survival

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