PNN Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from Mouse brain, using PNN Antibody.

Product:	PNN Antibody
Catalog:	DF4296
Description:	Rabbit polyclonal antibody to PNN
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	85 KD; 82kD(Calculated).
Uniprot:	Q9H307
RRID:	AB_2836647

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PNN Antibody detects endogenous levels of total PNN.

RRID:

AB_2836647
Cite Format: Affinity Biosciences Cat# DF4296, RRID:AB_2836647.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

140 kDa nuclear and cell adhesion-related phosphoprotein; Desmosome-associated protein; Domain-rich serine protein; DRS; DRS protein; DRSP; Melanoma metastasis clone A protein; MEMA; Nuclear protein SDK3; Pinin; PININ_HUMAN; PNN; SR like protein; SR-like protein;

Immunogens

Immunogen:

Uniprot:

Q9H307(PININ_HUMAN) >>Visit HPA database.

Gene(ID):

PNN(5411)

Expression:

Q9H307 PININ_HUMAN:

Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.

Sequence:

Q9H307 PININ_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGGGPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQRNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLEQKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINKMEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAEKEQEEEEQKQEMEVKMEEETEVRESEKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENEASKELEPEMEFEIEPDKECKTLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQSQPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRSRSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDSSSSTSSSSESRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKRSSRSERDRKSDRKDKRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H307 As Substrate

Q9H307

Site	PTM Type	Source
A2	Acetylation	Uniprot
T30	Phosphorylation	Uniprot
S48	Phosphorylation	Uniprot
R54	Methylation	Uniprot
R56	Methylation	Uniprot
S58	Phosphorylation	Uniprot
R62	Methylation	Uniprot
R63	Methylation	Uniprot
S66	Phosphorylation	Uniprot
S68	Phosphorylation	Uniprot
S84	Phosphorylation	Uniprot
T92	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
S100	Phosphorylation	Uniprot
K109	Sumoylation	Uniprot
K109	Ubiquitination	Uniprot
S114	Phosphorylation	Uniprot
S115	Phosphorylation	Uniprot
S120	Phosphorylation	Uniprot
K121	Ubiquitination	Uniprot
T124	Phosphorylation	Uniprot
K137	Ubiquitination	Uniprot
T152	Phosphorylation	Uniprot
K157	Methylation	Uniprot
K157	Sumoylation	Uniprot
K157	Ubiquitination	Uniprot
S160	Phosphorylation	Uniprot
T164	Phosphorylation	Uniprot
K212	Ubiquitination	Uniprot
K228	Ubiquitination	Uniprot
K238	Acetylation	Uniprot
K238	Ubiquitination	Uniprot
Y243	Phosphorylation	Uniprot
K262	Ubiquitination	Uniprot
K280	Ubiquitination	Uniprot
S289	Phosphorylation	Uniprot
K304	Sumoylation	Uniprot
S347	Phosphorylation	Uniprot
S375	Phosphorylation	Uniprot
S381	Phosphorylation	Uniprot
T408	Phosphorylation	Uniprot
S413	Phosphorylation	Uniprot
S417	Phosphorylation	Uniprot
S422	Phosphorylation	Uniprot
S443	Phosphorylation	Uniprot
S450	Phosphorylation	Uniprot
S458	Phosphorylation	Uniprot
K528	Ubiquitination	Uniprot
K536	Ubiquitination	Uniprot
T547	Phosphorylation	Uniprot
S552	Phosphorylation	Uniprot
K553	Sumoylation	Uniprot
T558	Phosphorylation	Uniprot
S560	Phosphorylation	Uniprot
S575	Phosphorylation	Uniprot
S578	Phosphorylation	Uniprot
S579	Phosphorylation	Uniprot
S580	Phosphorylation	Uniprot
S581	Phosphorylation	Uniprot
S582	Phosphorylation	Uniprot
S583	Phosphorylation	Uniprot
S587	Phosphorylation	Uniprot
S589	Phosphorylation	Uniprot
S590	Phosphorylation	Uniprot
S591	Phosphorylation	Uniprot
S594	Phosphorylation	Uniprot
S597	Phosphorylation	Uniprot
S600	Phosphorylation	Uniprot
S601	Phosphorylation	Uniprot
S602	Phosphorylation	Uniprot
S603	Phosphorylation	Uniprot
R604	Methylation	Uniprot
S605	Phosphorylation	Uniprot
S611	Phosphorylation	Uniprot
S618	Phosphorylation	Uniprot
S621	Phosphorylation	Uniprot
S623	Phosphorylation	Uniprot
S624	Phosphorylation	Uniprot
S629	Phosphorylation	Uniprot
S633	Phosphorylation	Uniprot
S649	Phosphorylation	Uniprot
T657	Phosphorylation	Uniprot
S658	Phosphorylation	Uniprot
S663	Phosphorylation	Uniprot
S666	Phosphorylation	Uniprot
S667	Phosphorylation	Uniprot
K668	Acetylation	Uniprot
S671	Phosphorylation	Uniprot
S672	Phosphorylation	Uniprot
S690	Phosphorylation	Uniprot
S692	Phosphorylation	Uniprot
S694	Phosphorylation	Uniprot
S695	Phosphorylation	Uniprot
S697	Phosphorylation	Uniprot
S702	Phosphorylation	Uniprot
S704	Phosphorylation	Uniprot

Research Backgrounds

Q9H307_PININ_HUMAN

Function:

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

Subcellular Location:

Nucleus speckle. Cell junction>Desmosome.
Note: Cell-cell contact area, predominantly desmosome of intercellular adherens junction. Not a nucleocytoplasmic shuttling protein.

Tissue Specificity:

Subunit Structure:

Found in a mRNA splicing-dependent exon junction complex (EJC). Found in a complex with SR proteins. Found in a mRNP complex with RNPS1. Component of the PSAP complex consisting of RNPS1, SAP18 and PNN. Interacts with PNISR, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 and SRRM2. Identified in the spliceosome C complex.

Family&Domains:

Belongs to the pinin family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PNN Antibody - #DF4296