PIGX Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from COLO cells using PIGX antibody.

Product:	PIGX Antibody
Catalog:	DF4293
Description:	Rabbit polyclonal antibody to PIGX
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Dog
Mol.Wt.:	26 KD; 29kD(Calculated).
Uniprot:	Q8TBF5
RRID:	AB_2836644

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(91%), Horse(82%), Dog(82%)

Clonality:

Polyclonal

Specificity:

PIGX Antibody detects endogenous levels of total PIGX.

RRID:

AB_2836644
Cite Format: Affinity Biosciences Cat# DF4293, RRID:AB_2836644.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GPI mannosyltransferase subunit; Phosphatidylinositol glycan class X; Phosphatidylinositol-glycan biosynthesis class X protein; PIG-X; Pigx; PIGX_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q8TBF5(PIGX_HUMAN) >>Visit HPA database.

Gene(ID):

PIGX(54965)

Sequence:

Q8TBF5 PIGX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAARVAAVRAAAWLLLGAATGLTRGPAAAFTAARSDAGIRAMCSEIILRQEVLKDGFHRDLLIKVKFGESIEDLHTCRLLIKQDIPAGLYVDPYELASLRERNITEAVMVSENFDIEAPNYLSKESEVLIYARRDSQCIDCFQAFLPVHCRYHRPHSEDGEASIVVNNPDLLMFCDQEFPILKCWAHSEVAAPCALENEDICQWNKMKYKSVYKNVILQVPVGLTVHTSLVCSVTLLITILCSTLILVAVFKYGHFSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Horse

Dog

Rabbit

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TBF5 As Substrate

Q8TBF5

Site	PTM Type	Source
T31	O-Glycosylation	Uniprot
K54	Ubiquitination	Uniprot
K66	Ubiquitination	Uniprot
K82	Ubiquitination	Uniprot

Research Backgrounds

Q8TBF5_PIGX_HUMAN

Function:

Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM (By similarity).

PTMs:

N-glycosylated.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type I membrane protein.

Subunit Structure:

Interacts with PIGM.

Family&Domains:

Belongs to the PIGX family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosylphosphatidylinositol (GPI)-anchor biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PIGX Antibody - #DF4293