PEX7 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat muscle, using PEX7 Antibody.

Product:	PEX7 Antibody
Catalog:	DF4286
Description:	Rabbit polyclonal antibody to PEX7
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	40 KD; 36kD(Calculated).
Uniprot:	O00628
RRID:	AB_2836637

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Zebrafish(86%), Bovine(86%), Horse(86%), Sheep(86%), Rabbit(100%), Dog(86%)

Clonality:

Polyclonal

Specificity:

PEX7 Antibody detects endogenous levels of total PEX7.

RRID:

AB_2836637
Cite Format: Affinity Biosciences Cat# DF4286, RRID:AB_2836637.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

PBD9B; PCDP1; Peroxin 7; Peroxin-7; Peroxisomal PTS2 receptor; Peroxisomal targeting signal 2 receptor; Peroxisome biogenesis factor 7; Peroxisome targeting signal 2 receptor; PEX7; PEX7 protein; PEX7_HUMAN; PTS2 receptor; PTS2R; RCDP1; RD;

Immunogens

Immunogen:

Uniprot:

O00628(PEX7_HUMAN) >>Visit HPA database.

Gene(ID):

PEX7(5191)

Expression:

O00628 PEX7_HUMAN:

Ubiquitous. Highest expression in pancreas, skeletal muscle and heart.

Sequence:

O00628 PEX7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSAVCGGAARMLRTPGRHGYAAEFSPYLPGRLACATAQHYGIAGCGTLLILDPDEAGLRLFRSFDWNDGLFDVTWSENNEHVLITCSGDGSLQLWDTAKAAGPLQVYKEHAQEVYSVDWSQTRGEQLVVSGSWDQTVKLWDPTVGKSLCTFRGHESIIYSTIWSPHIPGCFASASGDQTLRIWDVKAAGVRIVIPAHQAEILSCDWCKYNENLLVTGAVDCSLRGWDLRNVRQPVFELLGHTYAIRRVKFSPFHASVLASCSYDFTVRFWNFSKPDSLLETVEHHTEFTCGLDFSLQSPTQVADCSWDETIKIYDPACLTIPA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00628 As Substrate

O00628

Site	PTM Type	Source
K138	Acetylation	Uniprot
K186	Ubiquitination	Uniprot
S256	Phosphorylation	Uniprot
S260	Phosphorylation	Uniprot

Research Backgrounds

O00628_PEX7_HUMAN

Function:

Binds to the N-terminal PTS2-type peroxisomal targeting signal and plays an essential role in peroxisomal protein import.

Subcellular Location:

Peroxisome. Cytoplasm.

Tissue Specificity:

Ubiquitous. Highest expression in pancreas, skeletal muscle and heart.

Subunit Structure:

Interacts with PEX5 (By similarity). Interacts with VWA8.

Family&Domains:

Belongs to the WD repeat peroxin-7 family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

PEX7 Antibody - #DF4286