PEX14 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using PEX14 Antibody.

Product:	PEX14 Antibody
Catalog:	DF4284
Description:	Rabbit polyclonal antibody to PEX14
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
Mol.Wt.:	38 KD; 41kD(Calculated).
Uniprot:	O75381
RRID:	AB_2836635

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(85%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

PEX14 Antibody detects endogenous levels of total PEX14.

RRID:

AB_2836635
Cite Format: Affinity Biosciences Cat# DF4284, RRID:AB_2836635.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

dJ734G22.2; NAPP2; NF E2 associated polypeptide 2; Peroxin 14; Peroxin-14; Peroxisomal biogenesis factor 14; Peroxisomal membrane anchor protein PEX14; Peroxisomal membrane anchor protein Pex14p; Peroxisomal membrane protein PEX14; pex14; PEX14_HUMAN; Pex14p; PTS1 receptor docking protein; PTS1 receptor-docking protein;

Immunogens

Immunogen:

Uniprot:

O75381(PEX14_HUMAN) >>Visit HPA database.

Gene(ID):

PEX14(5195)

Sequence:

O75381 PEX14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASSEQAEQPSQPSSTPGSENVLPREPLIATAVKFLQNSRVRQSPLATRRAFLKKKGLTDEEIDMAFQQSGTAADEPSSLGPATQVVPVQPPHLISQPYSPAGSRWRDYGALAIIMAGIAFGFHQLYKKYLLPLILGGREDRKQLERMEAGLSELSGSVAQTVTQLQTTLASVQELLIQQQQKIQELAHELAAAKATTSTNWILESQNINELKSEINSLKGLLLNRRQFPPSPSAPKIPSWQIPVKSPSPSSPAAVNHHSSSDISPVSNESTSSSPGKEGHSPEGSTVTYHLLGPQEEGEGVVDVKGQVRMEVQGEEEKREDKEDEEDEEDDDVSHVDEEDCLGVQREDRRGGDGQINEQVEKLRRPEGASNESERD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Xenopus

Zebrafish

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75381 As Substrate

O75381

Site	PTM Type	Source
A2	Acetylation	Uniprot
T16	Phosphorylation	Uniprot
K34	Acetylation	Uniprot
K34	Ubiquitination	Uniprot
S44	Phosphorylation	Uniprot
K195	Ubiquitination	Uniprot
K213	Ubiquitination	Uniprot
K220	Ubiquitination	Uniprot
S232	Phosphorylation	Uniprot
S234	Phosphorylation	Uniprot
K246	Ubiquitination	Uniprot
S249	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
S260	Phosphorylation	Uniprot
S261	Phosphorylation	Uniprot
S262	Phosphorylation	Uniprot
S265	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S282	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
T289	Phosphorylation	Uniprot
Y290	Phosphorylation	Uniprot
S335	Phosphorylation	Uniprot
R351	Methylation	Uniprot
K363	Ubiquitination	Uniprot
S374	Phosphorylation	Uniprot

Research Backgrounds

O75381_PEX14_HUMAN

Function:

Peroxisome membrane protein that is an essential component of the peroxisomal import machinery. Functions as a docking factor for the predominantly cytoplasmic PTS1 receptor (PEX5). Plays a key role for peroxisome movement through a direct interaction with tubulin.

Subcellular Location:

Peroxisome membrane>Peripheral membrane protein>Cytoplasmic side.

Subunit Structure:

Interacts with PEX5, PEX13 and PEX19. Interacts with tubulin.

Family&Domains:

Belongs to the peroxin-14 family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

Restrictive clause

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PEX14 Antibody - #DF4284