Product: PEX3 Antibody
Catalog: DF4282
Description: Rabbit polyclonal antibody to PEX3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 42 KD; 42kD(Calculated).
Uniprot: P56589
RRID: AB_2836633

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
PEX3 Antibody detects endogenous levels of total PEX3.
RRID:
AB_2836633
Cite Format: Affinity Biosciences Cat# DF4282, RRID:AB_2836633.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Peroxin-3; Peroxisomal assembly protein PEX3; Peroxisomal biogenesis factor 3; PEX3; PEX3_HUMAN; TRG18;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P56589 PEX3_HUMAN:

Found in all examined tissues.

Sequence:
MLRSVWNFLKRHKKKCIFLGTVLGGVYILGKYGQKKIREIQEREAAEYIAQARRQYHFESNQRTCNMTVLSMLPTLREALMQQLNSESLTALLKNRPSNKLEIWEDLKIISFTRSTVAVYSTCMLVVLLRVQLNIIGGYIYLDNAAVGKNGTTILAPPDVQQQYLSSIQHLLGDGLTELITVIKQAVQKVLGSVSLKHSLSLLDLEQKLKEIRNLVEQHKSSSWINKDGSKPLLCHYMMPDEETPLAVQACGLSPRDITTIKLLNETRDMLESPDFSTVLNTCLNRGFSRLLDNMAEFFRPTEQDLQHGNSMNSLSSVSLPLAKIIPIVNGQIHSVCSETPSHFVQDLLTMEQVKDFAANVYEAFSTPQQLEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Horse
90
Rabbit
90
Xenopus
80
Zebrafish
60
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56589 As Substrate

Site PTM Type Enzyme
Y27 Phosphorylation
Y48 Phosphorylation
T75 Phosphorylation
K100 Ubiquitination
K189 Ubiquitination
K197 Ubiquitination
S201 Phosphorylation
K208 Ubiquitination
K220 Ubiquitination
S221 Phosphorylation
K227 Ubiquitination
S254 Phosphorylation
T260 Phosphorylation
K262 Ubiquitination
S311 Phosphorylation

Research Backgrounds

Function:

Involved in peroxisome biosynthesis and integrity. Assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, is necessary for the import of peroxisomal membrane proteins in the peroxisomes.

Subcellular Location:

Peroxisome membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in all examined tissues.

Subunit Structure:

Interacts with PEX19.

Family&Domains:

Belongs to the peroxin-3 family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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