PEX19 Antibody - #DF4281

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Product: PEX19 Antibody
Catalog: DF4281
Description: Rabbit polyclonal antibody to PEX19
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 33 KD; 33kD(Calculated).
Uniprot: P40855
RRID: AB_2836632

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
PEX19 Antibody detects endogenous levels of total PEX19.
RRID:
AB_2836632
Cite Format: Affinity Biosciences Cat# DF4281, RRID:AB_2836632.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

33 kDa housekeeping protein; D1S2223E; HK33; Housekeeping gene 33kD; OK/SW-cl.22; PBD12A; Peroxin 19; Peroxin-19; Peroxisomal biogenesis factor 19; Peroxisomal farnesylated protein; PEX19; PEX19_HUMAN; PMP1; PMPI; PXF; PXMP1;

Immunogens

Immunogen:
Uniprot:

P40855(PEX19_HUMAN) >>Visit HPA database.

Gene(ID):
PEX19(5824)
Expression:
P40855 PEX19_HUMAN:

Ubiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form.

Sequence:
MAAAEEGCSVGAEADRELEELLESALDDFDKAKPSPAPPSTTTAPDASGPQKRSPGDTAKDALFASQEKFFQELFDSELASQATAEFEKAMKELAEEEPHLVEQFQKLSEAAGRVGSDMTSQQEFTSCLKETLSGLAKNATDLQNSSMSEEELTKAMEGLGMDEGDGEGNILPIMQSIMQNLLSKDVLYPSLKEITEKYPEWLQSHRESLPPEQFEKYQEQHSVMCKICEQFEAETPTDSETTQKARFEMVLDLMQQLQDLGHPPKELAGEMPPGLNFDLDALNLSGPPGASGEQCLIM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Dog
89
Sheep
78
Rabbit
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40855 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S9 Phosphorylation
K33 Ubiquitination
S35 Phosphorylation
S40 Phosphorylation
T42 Phosphorylation
K52 Ubiquitination
S54 Phosphorylation
T58 Phosphorylation
K60 Ubiquitination
S66 Phosphorylation
K69 Ubiquitination
K89 Ubiquitination
K92 Ubiquitination
K107 Ubiquitination
S117 Phosphorylation
K130 Ubiquitination
S134 Phosphorylation
K138 Ubiquitination
T141 Phosphorylation
S146 Phosphorylation
S147 Phosphorylation
S149 Phosphorylation
S177 Phosphorylation
S184 Phosphorylation
K193 Ubiquitination
K198 Ubiquitination
Y199 Phosphorylation
T236 Phosphorylation
K245 Ubiquitination

Research Backgrounds

Function:

Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.

Subcellular Location:

Cytoplasm. Peroxisome membrane>Lipid-anchor>Cytoplasmic side.
Note: Mainly cytoplasmic. Some fraction membrane-associated to the outer surface of peroxisomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Isoform 1 is strongly predominant in all tissues except in utero where isoform 2 is the main form.

Subunit Structure:

Interacts with a broad range of peroxisomal membrane proteins, including PEX3, PEX10, PEX11A, PEX11B, PEX12, PEX13, PEX14 and PEX16, PXMP2/PMP22, PXMP4/PMP24, SLC25A17/PMP34, ABCD1/ALDP, ABCD2/ALDRP, and ABCD3/PMP70. Also interacts with the tumor suppressor CDKN2A/p19ARF.

Family&Domains:

Belongs to the peroxin-19 family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

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