PARD6B Antibody - #DF4277

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Product: PARD6B Antibody
Catalog: DF4277
Description: Rabbit polyclonal antibody to PARD6B
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 38 KD; 41kD(Calculated).
Uniprot: Q9BYG5
RRID: AB_2836628

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
PARD6B Antibody detects endogenous levels of total PARD6B.
RRID:
AB_2836628
Cite Format: Affinity Biosciences Cat# DF4277, RRID:AB_2836628.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000031269; PAR 6 beta; Par 6 partitioning defective 6 homolog beta (C. elegans); PAR6B; PARD6 beta; PARD6B; partitioning defective 6 homolog beta; partitioning-defective protein 6, C. elegans, homolog of, beta;

Immunogens

Immunogen:
Uniprot:

Q9BYG5(PAR6B_HUMAN) >>Visit HPA database.

Gene(ID):
PARD6B(84612)
Expression:
Q9BYG5 PAR6B_HUMAN:

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Sequence:
MNRSHRHGAGSGCLGTMEVKSKFGAEFRRFSLERSKPGKFEEFYGLLQHVHKIPNVDVLVGYADIHGDLLPINNDDNYHKAVSTANPLLRIFIQKKEEADYSAFGTDTLIKKKNVLTNVLRPDNHRKKPHIVISMPQDFRPVSSIIDVDILPETHRRVRLYKYGTEKPLGFYIRDGSSVRVTPHGLEKVPGIFISRLVPGGLAQSTGLLAVNDEVLEVNGIEVSGKSLDQVTDMMIANSRNLIITVRPANQRNNVVRNSRTSGSSGQSTDNSLLGYPQQIEPSFEPEDEDSEEDDIIIEDNGVPQQIPKAVPNTESLESLTQIELSFESGQNGFIPSNEVSLAAIASSSNTEFETHAPDQKLLEEDGTIITL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
89
Xenopus
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BYG5 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S11 Phosphorylation
Y101 Phosphorylation
S102 Phosphorylation
T108 Phosphorylation
S143 Phosphorylation
S144 Phosphorylation
S177 Phosphorylation
K188 Ubiquitination
S291 Phosphorylation
S316 Phosphorylation
S349 Phosphorylation
T355 Phosphorylation
T371 Phosphorylation

Research Backgrounds

Function:

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Subcellular Location:

Cytoplasm. Cell membrane. Cell junction>Tight junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Subunit Structure:

Interacts with PARD3. Interacts with GTP-bound forms of CDC42 and RAC1. Interacts with GTP-bound ARHQ/TC10. Interacts with MPP5 (By similarity). Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with ALS2CR19. Interacts with ECT2.

Family&Domains:

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Belongs to the PAR6 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Development > Axon guidance.   (View pathway)

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