OAT Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from K562/COLO205 cells, using OAT antibody.

Western blot analysis of extracts from K562 cells, using OAT Antibody.

Product:	OAT Antibody
Catalog:	DF4270
Description:	Rabbit polyclonal antibody to OAT
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	48 KD; 49kD(Calculated).
Uniprot:	P04181
RRID:	AB_2836621

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(82%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Chicken(91%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

OAT Antibody detects endogenous levels of total OAT.

RRID:

AB_2836621
Cite Format: Affinity Biosciences Cat# DF4270, RRID:AB_2836621.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GACR; Gyrate atrophy; HOGA; OAT; OATASE; OKT; Ornithine aminotransferase (gyrate atrophy); Ornithine aminotransferase mitochondrial; ornithine aminotransferase precursor; Ornithine aminotransferase, mitochondrial precursor; Ornithine delta aminotransferase; Ornithine oxo acid aminotransferase;

Immunogens

Immunogen:

Uniprot:

P04181(OAT_HUMAN) >>Visit HPA database.

Gene(ID):

OAT(4942)

Sequence:

P04181 OAT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFSKLAHLQRFAVLSRGVHSSVASATSVATKKTVQGPPTSDDIFEREYKYGAHNYHPLPVALERGKGIYLWDVEGRKYFDFLSSYSAVNQGHCHPKIVNALKSQVDKLTLTSRAFYNNVLGEYEEYITKLFNYHKVLPMNTGVEAGETACKLARKWGYTVKGIQKYKAKIVFAAGNFWGRTLSAISSSTDPTSYDGFGPFMPGFDIIPYNDLPALERALQDPNVAAFMVEPIQGEAGVVVPDPGYLMGVRELCTRHQVLFIADEIQTGLARTGRWLAVDYENVRPDIVLLGKALSGGLYPVSAVLCDDDIMLTIKPGEHGSTYGGNPLGCRVAIAALEVLEEENLAENADKLGIILRNELMKLPSDVVTAVRGKGLLNAIVIKETKDWDAWKVCLRLRDNGLLAKPTHGDIIRFAPPLVIKEDELRESIEIINKTILSF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Xenopus

100

Chicken

Pig

Zebrafish

Rabbit

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04181 As Substrate

P04181

Site	PTM Type	Source
T30	Phosphorylation	Uniprot
K32	Ubiquitination	Uniprot
T39	Phosphorylation	Uniprot
K49	Ubiquitination	Uniprot
Y55	Phosphorylation	Uniprot
K66	Ubiquitination	Uniprot
Y69	Phosphorylation	Uniprot
Y85	Phosphorylation	Uniprot
K96	Acetylation	Uniprot
K102	Ubiquitination	Uniprot
Y123	Phosphorylation	Uniprot
Y126	Phosphorylation	Uniprot
Y133	Phosphorylation	Uniprot
K151	Ubiquitination	Uniprot
T272	Phosphorylation	Uniprot
Y280	Phosphorylation	Uniprot
C306	S-Nitrosylation	Uniprot
C330	S-Nitrosylation	Uniprot
K362	Ubiquitination	Uniprot
S365	Phosphorylation	Uniprot
K374	Ubiquitination	Uniprot
K386	Ubiquitination	Uniprot
K392	Ubiquitination	Uniprot
K405	Ubiquitination	Uniprot
R426	Methylation	Uniprot

Research Backgrounds

P04181_OAT_HUMAN

Subcellular Location:

Mitochondrion matrix.

Subunit Structure:

Homohexamer.

Family&Domains:

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Diterpenoid alkaloids isolated from Delphinium trichophorum alleviate pulmonary fibrosis via the TGF-β/Smad pathway in 3T6 and HFL-1 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 36068772) [IF=7.5]

2). Inactivation of PDH can Reduce Anaplastic Thyroid Cancer Cells' Sensitivity to Artemisinin. Anti-Cancer Agents in Medicinal Chemistry, 2022 (PubMed: 34515013) [IF=2.8]

Restrictive clause

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OAT Antibody - #DF4270