NUSAP1 Antibody - #DF4261

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Product: NUSAP1 Antibody
Catalog: DF4261
Description: Rabbit polyclonal antibody to NUSAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 49 KD; 49kD(Calculated).
Uniprot: Q9BXS6
RRID: AB_2836612

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
NUSAP1 Antibody detects endogenous levels of total NUSAP1.
RRID:
AB_2836612
Cite Format: Affinity Biosciences Cat# DF4261, RRID:AB_2836612.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2610201A12Rik; AI481307; ANKT; AW547774; BB165529; BM037; FLJ13421; LNP; Nucleolar and spindle associated protein 1; Nucleolar and spindle-associated protein 1; Nucleolar protein ANKT; NuSAP; NUSAP_HUMAN; nusap1; PRO0310p1; Q0310; RP23-22A15.4; SAPL;

Immunogens

Immunogen:
Uniprot:

Q9BXS6(NUSAP_HUMAN) >>Visit HPA database.

Gene(ID):
NUSAP1(51203)
Sequence:
MIIPSLEELDSLKYSDLQNLAKSLGLRANLRATKLLKALKGYIKHEARKGNENQDESQTSASSCDETEIQISNQEEAERQPLGHVTKTRRRCKTVRVDPDSQQNHSEIKISNPTEFQNHEKQESQDLRATAKVPSPPDEHQEAENAVSSGNRDSKVPSEGKKSLYTDESSKPGKNKRTAITTPNFKKLHEAHFKEMESIDQYIERKKKHFEEHNSMNELKQQPINKGGVRTPVPPRGRLSVASTPISQRRSQGRSCGPASQSTLGLKGSLKRSAISAAKTGVRFSAATKDNEHKRSLTKTPARKSAHVTVSGGTPKGEAVLGTHKLKTITGNSAAVITPFKLTTEATQTPVSNKKPVFDLKASLSRPLNYEPHKGKLKPWGQSKENNYLNQHVNRINFYKKTYKQPHLQTKEEQRKKREQERKEKKAKVLGMRRGLILAED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Chicken
82
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXS6 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
T33 Phosphorylation
S101 Phosphorylation
S106 Phosphorylation
S124 Phosphorylation Q13315 (ATM)
S135 Phosphorylation
S148 Phosphorylation
S149 Phosphorylation
S154 Phosphorylation
S158 Phosphorylation
Y165 Phosphorylation
S169 Phosphorylation
S170 Phosphorylation
T181 Phosphorylation
T182 Phosphorylation
K186 Methylation
K187 Ubiquitination
S215 Phosphorylation
T231 Phosphorylation
S240 Phosphorylation Q96GD4 (AURKB) , O14965 (AURKA)
S243 Phosphorylation
T244 Phosphorylation
S247 Phosphorylation
S251 Phosphorylation
S255 Phosphorylation
C256 S-Nitrosylation
S260 Phosphorylation
S262 Phosphorylation
T263 Phosphorylation
K267 Methylation
S269 Phosphorylation
K271 Methylation
S273 Phosphorylation
S276 Phosphorylation
K279 Ubiquitination
S285 Phosphorylation
K289 Acetylation
S296 Phosphorylation
T298 Phosphorylation
T300 Phosphorylation P06493 (CDK1)
K304 Ubiquitination
S305 Phosphorylation
S311 Phosphorylation
T314 Phosphorylation
K316 Ubiquitination
S333 Phosphorylation
T338 Phosphorylation P06493 (CDK1)
T343 Phosphorylation
T344 Phosphorylation
T347 Phosphorylation
T349 Phosphorylation
S352 Phosphorylation
K355 Ubiquitination
K361 Ubiquitination
S363 Phosphorylation
S365 Phosphorylation
K376 Ubiquitination
K378 Ubiquitination
K384 Methylation
Y388 Phosphorylation
K404 Ubiquitination
K411 Acetylation

Research Backgrounds

Function:

Microtubule-associated protein with the capacity to bundle and stabilize microtubules (By similarity). May associate with chromosomes and promote the organization of mitotic spindle microtubules around them.

PTMs:

Ubiquitinated. Ubiquitination by FZR1 may lead to proteasome-dependent degradation of this protein.

Phosphorylation by ATM in G2/M-phase induces mitotic arrest.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus. Cytoplasm>Cytoskeleton>Spindle. Chromosome.
Note: Found in the cytoplasm and nucleolus during interphase and redistributes to the mitotic spindle in prometaphase (By similarity). Localizes to the mitotic spindle during anaphase and telophase then disappears from around the chromosomes during cytokinesis (By similarity). Localizes to multiple distinct regions of chromosomes throughout mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with DNA and microtubules. Microtubule bundling is inhibited by IPO7, KPNA2 and KPNB1 while association with DNA is also inhibited by IPO7 and KPNA2 (By similarity).

Family&Domains:

The KEN box is required for the FZR1-dependent degradation of this protein subsequent to ubiquitination.

Belongs to the NUSAP family.

References

1). Sharp Downregulation of Hub Genes Associated With the Pathogenesis of Breast Cancer From Ductal Carcinoma In Situ to Invasive Ductal Carcinoma. Frontiers in Oncology, 2021 (PubMed: 34094915) [IF=3.5]

Application: WB    Species: Human    Sample: breast cancer tissue

Figure 6 Protein expression levels of hub genes. (A) Protein expression levels of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 were determined by western blotting. (B) Western blot analysis of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 in tissue from different stages of breast disease, and quantification of the intensity relative to GAPDH. One-way ANOVA was performed to acquire statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). NME, normal mammary epithelium; SH, simple ductal hyperplasia; ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; IDC, invasive ductal carcinoma.

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