NXF3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from A375 cells, using NXF3 Antibody.

Product:	NXF3 Antibody
Catalog:	DF4258
Description:	Rabbit polyclonal antibody to NXF3
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Dog
Mol.Wt.:	60 KD; 60kD(Calculated).
Uniprot:	Q9H4D5
RRID:	AB_2836609

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Dog(80%)

Clonality:

Polyclonal

Specificity:

NXF3 Antibody detects endogenous levels of total NXF3.

RRID:

AB_2836609
Cite Format: Affinity Biosciences Cat# DF4258, RRID:AB_2836609.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Nuclear RNA export factor 3; NXF3; NXF3_HUMAN; OTTHUMP00000023726; OTTHUMP00000023727; TAP like protein 3; TAP-like protein 3; TAPL 3; TAPL-3; TAPL3;

Immunogens

Immunogen:

Uniprot:

Q9H4D5(NXF3_HUMAN) >>Visit HPA database.

Gene(ID):

NXF3(56000)

Expression:

Q9H4D5 NXF3_HUMAN:

Expressed at high level in testis and at low level in a small number of tissues.

Sequence:

Q9H4D5 NXF3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLPSGHTTGHTDQVVQRRARCWDIYQRRFSSRSEPVNPGMHSSSHQQQDGDAAMHGAHMDSPVRYTPYTISPYNRKGSFRKQDQTHVNMEREQKPPERRMEGNMPDGTLGSWFKITVPFGIKYNEKWLLNLIQNECSVPFVPVEFHYENMHASFFVENASIAYALKNVSGKIWDEDNEKISIFVNPAGIPHFVHRELKSEKVEQIKLAMNQQCDVSQEALDIQRLPFYPDMVNRDTKMASNPRKCMAASLDVHEENIPTVMSAGEMDKWKGIEPGEKCADRSPVCTTFSDTSSNINSILELFPKLLCLDGQQSPRATLCGTEAHKRLPTCKGSFFGSEMLKNLVLQFLQQYYLIYDSGDRQGLLSAYHDEACFSLSIPFNPEDSAPSSFCKFFKDSRNIKILKDPYLRGELLKHTKLDIVDSLSALPKTQHDLSSFLVDMWYQTEWMLCFSVNGVFKEVEGQSQGSVLAFTRTFIATPGSSSSLCIVNDKLFVRDTSHQGTQSALFTLVPTAFSSSVPAFSQEQQKMLPS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Rabbit

Bovine

Pig

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H4D5 As Substrate

Q9H4D5

Site	PTM Type	Enzyme	Source
Y124	Phosphorylation		Uniprot
Y407	Phosphorylation		Uniprot

Research Backgrounds

Q9H4D5_NXF3_HUMAN

Function:

May function as a tissue-specific nuclear mRNA export factor.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm.

Tissue Specificity:

Expressed at high level in testis and at low level in a small number of tissues.

Subunit Structure:

Interacts with NXT1, NXT2, E1B-AP5 and CRM1 nuclear export factor.

Family&Domains:

Lacks C-terminal domain that mediates direct interactions with nucleoporins.

Contains a novel CRM1-dependent nuclear export signal that compensates in cis for the loss of the nuclear pore targeting domain.

The RNA-binding domain is a non-canonical RNP-type domain.

Belongs to the NXF family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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NXF3 Antibody - #DF4258