NRBF2 Antibody - #DF4257

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Product: NRBF2 Antibody
Catalog: DF4257
Description: Rabbit polyclonal antibody to NRBF2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36 KD; 32kD(Calculated).
Uniprot: Q96F24
RRID: AB_2836608

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
NRBF2 Antibody detects endogenous levels of total NRBF2.
RRID:
AB_2836608
Cite Format: Affinity Biosciences Cat# DF4257, RRID:AB_2836608.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Comodulator of PPAR and RXR 1; Comodulator of PPAR and RXR 2; Comodulator of PPAR and RXR; COPR; COPR1; COPR2; DKFZp564C1664; FLJ30395; NRBF 2; NRBF-2; NRBF2; NRBF2_HUMAN; Nuclear receptor binding factor 2; Nuclear receptor-binding factor 2;

Immunogens

Immunogen:
Uniprot:

Q96F24(NRBF2_HUMAN) >>Visit HPA database.

Gene(ID):
NRBF2(29982)
Expression:
Q96F24 NRBF2_HUMAN:

Detected in keratinocytes, liver and placenta (PubMed:15610520). Expressed in a subset of cells in pediatric medulloblastoma (PubMed:18619852).

Sequence:
MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGFMNN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96F24 As Substrate

Site PTM Type Enzyme
K85 Acetylation
K103 Acetylation
S113 Phosphorylation
S116 Phosphorylation
K118 Ubiquitination
S120 Phosphorylation
Y143 Phosphorylation
K148 Ubiquitination
S149 Phosphorylation
S268 Phosphorylation
S277 Phosphorylation

Research Backgrounds

Function:

May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).

Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy. Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (By similarity). Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1. May be involved in autophagosome biogenesis. May play a role in neural progenitor cell survival during differentiation (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasmic vesicle. Cytoplasmic vesicle>Autophagosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in keratinocytes, liver and placenta. Expressed in a subset of cells in pediatric medulloblastoma.

Subunit Structure:

Interacts with PPARA, PPARD and PPARG. Interacts with RARA, RARG and RXRA in the presence of bound ligand. Interacts with SCOC. Associates with the PI3K complex I (PI3KC3-C1); the direct binding partner in the complex is reported variably as PIK3R4 or ATG14.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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