NUP93 Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat liver cells, using NUP93 antibody.

Product:	NUP93 Antibody
Catalog:	DF4252
Description:	Rabbit polyclonal antibody to NUP93
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	93 KD; 93kD(Calculated).
Uniprot:	Q8N1F7
RRID:	AB_2836603

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(89%)

Clonality:

Polyclonal

Specificity:

NUP93 Antibody detects endogenous levels of total NUP93.

RRID:

AB_2836603
Cite Format: Affinity Biosciences Cat# DF4252, RRID:AB_2836603.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2410008G02Rik; 93 kDa nucleoporin; CBP-interacting protein 4; Dead eye protein; Dye; KIAA0095; MGC21106; NIC96; NIC96, S. cerevisiae, homolog of; Nuclear pore complex protein Nup93; Nucleoporin 93; Nucleoporin 93kDa; Nucleoporin Nup93;

Immunogens

Immunogen:

Uniprot:

Q8N1F7(NUP93_HUMAN) >>Visit HPA database.

Gene(ID):

NUP93(9688)

Sequence:

Q8N1F7 NUP93_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDTEGFGELLQQAEQLAAETEGISELPHVERNLQEIQQAGERLRSRTLTRTSQETADVKASVLLGSRGLDISHISQRLESLSAATTFEPLEPVKDTDIQGFLKNEKDNALLSAIEESRKRTFGMAEEYHRESMLVEWEQVKQRILHTLLASGEDALDFTQESEPSYISDVGPPGRSSLDNIEMAYARQIYIYNEKIVNGHLQPNLVDLCASVAELDDKSISDMWTMVKQMTDVLLTPATDALKNRSSVEVRMEFVRQALAYLEQSYKNYTLVTVFGNLHQAQLGGVPGTYQLVRSFLNIKLPAPLPGLQDGEVEGHPVWALIYYCMRCGDLLAASQVVNRAQHQLGEFKTWFQEYMNSKDRRLSPATENKLRLHYRRALRNNTDPYKRAVYCIIGRCDVTDNQSEVADKTEDYLWLKLNQVCFDDDGTSSPQDRLTLSQFQKQLLEDYGESHFTVNQQPFLYFQVLFLTAQFEAAVAFLFRMERLRCHAVHVALVLFELKLLLKSSGQSAQLLSHEPGDPPCLRRLNFVRLLMLYTRKFESTDPREALQYFYFLRDEKDSQGENMFLRCVSELVIESREFDMILGKLENDGSRKPGVIDKFTSDTKPIINKVASVAENKGLFEEAAKLYDLAKNADKVLELMNKLLSPVVPQISAPQSNKERLKNMALSIAERYRAQGISANKFVDSTFYLLLDLITFFDEYHSGHIDRAFDIIERLKLVPLNQESVEERVAAFRNFSDEIRHNLSEVLLATMNILFTQFKRLKGTSPSSSSRPQRVIEDRDSQLRSQARTLITFAGMIPYRTSGDTNARLVQMEVLMN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Bovine

Sheep

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N1F7 As Substrate

Q8N1F7

Site	PTM Type	Source
T3	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
T47	Phosphorylation	Uniprot
T49	Phosphorylation	Uniprot
T51	Phosphorylation	Uniprot
S52	Phosphorylation	Uniprot
T55	Phosphorylation	Uniprot
K59	Ubiquitination	Uniprot
S66	Phosphorylation	Uniprot
R67	Methylation	Uniprot
S72	Phosphorylation	Uniprot
S75	Phosphorylation	Uniprot
S80	Phosphorylation	Uniprot
K94	Ubiquitination	Uniprot
K103	Acetylation	Uniprot
K103	Sumoylation	Uniprot
K103	Ubiquitination	Uniprot
K106	Acetylation	Uniprot
K106	Ubiquitination	Uniprot
S112	Phosphorylation	Uniprot
S117	Phosphorylation	Uniprot
K119	Ubiquitination	Uniprot
R120	Methylation	Uniprot
Y128	Phosphorylation	Uniprot
K141	Ubiquitination	Uniprot
T159	Phosphorylation	Uniprot
S162	Phosphorylation	Uniprot
S165	Phosphorylation	Uniprot
Y166	Phosphorylation	Uniprot
S168	Phosphorylation	Uniprot
Y185	Phosphorylation	Uniprot
Y190	Phosphorylation	Uniprot
T231	Phosphorylation	Uniprot
T236	Phosphorylation	Uniprot
K243	Ubiquitination	Uniprot
K349	Ubiquitination	Uniprot
T350	Phosphorylation	Uniprot
K359	Ubiquitination	Uniprot
S364	Phosphorylation	Uniprot
T367	Phosphorylation	Uniprot
K370	Ubiquitination	Uniprot
K387	Ubiquitination	Uniprot
Y391	Phosphorylation	Uniprot
K409	Ubiquitination	Uniprot
Y413	Phosphorylation	Uniprot
K417	Ubiquitination	Uniprot
S430	Phosphorylation	Uniprot
R434	Methylation	Uniprot
S438	Phosphorylation	Uniprot
T536	Phosphorylation	Uniprot
Y550	Phosphorylation	Uniprot
K558	Ubiquitination	Uniprot
S560	Phosphorylation	Uniprot
S571	Phosphorylation	Uniprot
K586	Ubiquitination	Uniprot
K594	Ubiquitination	Uniprot
K600	Ubiquitination	Uniprot
S603	Phosphorylation	Uniprot
K606	Sumoylation	Uniprot
K606	Ubiquitination	Uniprot
K611	Ubiquitination	Uniprot
S614	Phosphorylation	Uniprot
K619	Acetylation	Uniprot
K619	Ubiquitination	Uniprot
K627	Ubiquitination	Uniprot
K633	Ubiquitination	Uniprot
K637	Acetylation	Uniprot
K637	Ubiquitination	Uniprot
K644	Ubiquitination	Uniprot
S647	Phosphorylation	Uniprot
S654	Phosphorylation	Uniprot
S658	Phosphorylation	Uniprot
K660	Ubiquitination	Uniprot
K664	Ubiquitination	Uniprot
K718	Ubiquitination	Uniprot
S726	Phosphorylation	Uniprot
T758	Phosphorylation	Uniprot
K764	Ubiquitination	Uniprot
T766	Phosphorylation	Uniprot
S767	Phosphorylation	Uniprot
S770	Phosphorylation	Uniprot
S771	Phosphorylation	Uniprot
S772	Phosphorylation	Uniprot

Research Backgrounds

Q8N1F7_NUP93_HUMAN

Function:

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. May anchor nucleoporins, but not NUP153 and TPR, to the NPC. During renal development, regulates podocyte migration and proliferation through SMAD4 signaling.

Subcellular Location:

Nucleus membrane>Peripheral membrane protein. Nucleus>Nuclear pore complex. Nucleus envelope.
Note: Localizes at the nuclear basket and at or near the nuclear entry to the gated channel of the pore.

Subunit Structure:

Part of the nuclear pore complex (NPC). Component of the p62 complex, a complex composed of NUP62 and NUP54. Forms a complex with NUP35, NUP155, NUP205 and lamin B; the interaction with NUP35 is direct. Does not interact with TPR. Interacts with SMAD4 and IPO7; translocates SMAD4 to the nucleus through the NPC upon BMP7 stimulation resulting in activation of SMAD4 signaling.

Family&Domains:

Belongs to the nucleoporin interacting component (NIC) family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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NUP93 Antibody - #DF4252