VGF Antibody - #DF4244
| Product: | VGF Antibody |
| Catalog: | DF4244 |
| Description: | Rabbit polyclonal antibody to VGF |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Dog |
| Mol.Wt.: | 67,90kD; 67kD(Calculated). |
| Uniprot: | O15240 |
| RRID: | AB_2836595 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4244, RRID:AB_2836595.
Fold/Unfold
NERP-1; NERP-2; Nerve growth factor inducible; Neuroendocrine regulatory peptide-2; Neurosecretory protein VGF; VGF; VGF_HUMAN;
Immunogens
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
- O15240 VGF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKALRLSASALFCLLLINGLGAAPPGRPEAQPPPLSSEHKEPVAGDAVPGPKDGSAPEVRGARNSEPQDEGELFQGVDPRALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRPQTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASWGEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKARRPESALLGGSEAGERLLQQGLAQVEAGRRQAEATRQAAAQEERLADLASDLLLQYLLQGGARQRGLGGRGLQEAAEERESAREEEEAEQERRGGEERVGEEDEEAAEAEAEAEEAERARQNALLFAEEEDGEAGAEDKRSQEETPGHRRKEAEGTEEGGEEEDDEEMDPQTIDSLIELSTKLHLPADDVVSIIEEVEEKRKRKKNAPPEPVPPPRAAPAPTHVRSPQPPPPAPAPARDELPDWNEVLPPWDREEDEVYPPGPYHPFPNYIRPRTLQPPSALRRRHYHHALPPSRHYPGREAQARRAQEEAEAEERRLQEQEELENYIEHVLLRRP
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15240 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T180 | O-Glycosylation | Uniprot | |
| T187 | O-Glycosylation | Uniprot | |
| T189 | O-Glycosylation | Uniprot | |
| T191 | O-Glycosylation | Uniprot | |
| T193 | O-Glycosylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S252 | O-Glycosylation | Uniprot | |
| S253 | O-Glycosylation | Uniprot | |
| T256 | O-Glycosylation | Uniprot | |
| S266 | O-Glycosylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| T424 | Phosphorylation | Uniprot | |
| T554 | O-Glycosylation | Uniprot |
Research Backgrounds
Secreted polyprotein that is packaged and proteolytically processed by prohormone convertases PCSK1 and PCSK2 in a cell-type-specific manner (By similarity). VGF and peptides derived from its processing play many roles in neurogenesis and neuroplasticity associated with learning, memory, depression and chronic pain (By similarity).
Plays a role in the control of body fluid homeostasis by regulating vasopressin release. Suppresses presynaptic glutamatergic neurons connected to vasopressin neurons.
Plays a role in the control of body fluid homeostasis by regulating vasopressin release. Activates GABAergic interneurons which are inhibitory neurons of the nervous system and thereby suppresses presynaptic glutamatergic neurons (By similarity). Stimulates also feeding behavior in an orexin-dependent manner in the hypothalamus (By similarity). Functions as a positive regulator for the activation of orexin neurons resulting in elevated gastric acid secretion and gastric emptying (By similarity).
Secreted multifunctional neuropeptide that binds to different cell receptors and thereby plays multiple physiological roles including modulation of energy expenditure, pain, response to stress, gastric regulation, glucose homeostasis as well as lipolysis (By similarity). Activates the G-protein-coupled receptor C3AR1 via a folding-upon-binding mechanism leading to enhanced lipolysis in adipocytes (By similarity). Interacts with C1QBP receptor in macrophages and microglia causing increased levels of intracellular calcium and hypersensitivity (By similarity).
Plays a role in the regulation of memory formation and depression-related behaviors potentially by influencing synaptic plasticity and neurogenesis. Induces acute and transient activation of the NTRK2/TRKB receptor and subsequent CREB phosphorylation (By similarity). Induces also insulin secretion in insulinoma cells by increasing intracellular calcium mobilization (By similarity).
Has bactericidal activity against M. luteus, and antifungal activity against P. Pastoris.
Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
Secreted. Cytoplasmic vesicle>Secretory vesicle.
Note: Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
Interacts with HSPA8 on cell membrane. Interacts with C3AR1 (By similarity). Interacts with C1QBP (By similarity).
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