NMU Antibody - #DF4238

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Product: NMU Antibody
Catalog: DF4238
Description: Rabbit polyclonal antibody to NMU
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Zebrafish, Bovine, Xenopus
Mol.Wt.: 22 KD; 20kD(Calculated).
Uniprot: P48645
RRID: AB_2836589

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Zebrafish(100%), Bovine(89%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NMU Antibody detects endogenous levels of total NMU.
RRID:
AB_2836589
Cite Format: Affinity Biosciences Cat# DF4238, RRID:AB_2836589.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Neuromedin U 25; Neuromedin U; Neuromedin-U-25; NmU 25; NMU; NmU-25; NMU_HUMAN;

Immunogens

Immunogen:
Uniprot:

P48645(NMU_HUMAN) >>Visit HPA database.

Gene(ID):
NMU(10874)
Expression:
P48645 NMU_HUMAN:

Expressed throughout the enteric nervous system with highest levels being found in the jejunum.

Sequence:
MLRTESCRPRSPAGQVAAASPLLLLLLLLAWCAGACRGAPILPQGLQPEQQLQLWNEIDDTCSSFLSIDSQPQASNALEELCFMIMGMLPKPQEQDEKDNTKRFLFHYSKTQKLGKSNVVSSVVHPLLQLVPHLHERRMKRFRVDEEFQSPFASQSRGYFLFRPRNGRRSAGFI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Xenopus
100
Bovine
89
Pig
0
Horse
0
Sheep
0
Dog
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48645 As Substrate

Site PTM Type Enzyme
S109 O-Glycosylation

Research Backgrounds

Function:

Ligand for receptors NMUR1 and NMUR2 (By similarity). Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder.

Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release although its potency is much lower than that of neuromedin precursor-related peptide 36.

Does not function as a ligand for either NMUR1 or NMUR2. Indirectly induces prolactin release from lactotroph cells in the pituitary gland, probably via the hypothalamic dopaminergic system.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed throughout the enteric nervous system with highest levels being found in the jejunum.

Family&Domains:

Belongs to the NmU family.

References

1). Expression of NMU, PPBP and GNG4 in colon cancer and their influences on prognosis. Translational Cancer Research, 2022 (PubMed: 36388046) [IF=1.5]

Application: WB    Species: Human    Sample: colon cancer cells

Figure 3 Expression of key genes in colon cancer cells. (A) NMU, GNG4, PPBP and AGT mRNA levels were detected by RT-qPCR. (B) The expression of NMU, GNG4 and PPBP was detected by immunoblotting. *, P
2). NMU Is a Poor Prognostic Biomarker in Patients with Lung Adenocarcinoma. DISEASE MARKERS, 2021 (PubMed: 34336003)

Application: WB    Species: Human    Sample: LUAD cells and lung normal cells

Figure 6 The high NMU expression in LUAD cell lines. (a) qRT-PCR detection of the levels of NMU in LUAD cells and lung normal cells. (b) Western blot analysis to detect the expression of NMU protein in LUAD cells and lung normal cells. (c) The bar chart showing the ratio of the NMU protein to actin by densitometry in the different cells. (∗∗∗p < 0.001 vs. normal 16HBE cell).

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