NBPF1/9/10/12/14/15/16/20 Antibody - #DF4233
(3)
| Product: | NBPF1/9/10/12/14/15/16/20 Antibody |
| Catalog: | DF4233 |
| Description: | Rabbit polyclonal antibody to NBPF1/9/10/12/14/15/16/20 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 36 KD; 139kD,168kD,106kD,96kD,78kD(Calculated). |
| Uniprot: | Q3BBV0 | Q5TAG4 | Q5TI25 | Q6P3W6 | Q8N660 |
| RRID: | AB_2836584 |
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NBPF1/9/10/12/14/15/16/20 Antibody detects endogenous levels of total NBPF1/9/10/12/14/15/16/20.
RRID:
AB_2836584
Cite Format: Affinity Biosciences Cat# DF4233, RRID:AB_2836584.
Cite Format: Affinity Biosciences Cat# DF4233, RRID:AB_2836584.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
NBPF1
Immunogens
Immunogen:
Uniprot:
Q3BBV0(NBPF1_HUMAN) >>Visit HPA database.
Q5TAG4(NBPFC_HUMAN) >>Visit HPA database.
Q5TI25(NBPFE_HUMAN) >>Visit HPA database.
Gene(ID):
Expression:
Q3BBV0 NBPF1_HUMAN:
Widely expressed. The only tissue which shows a weak expression is kidney.
Q5TAG4 NBPFC_HUMAN:Widely expressed with highest levels in brain, ovary, mammary gland, skin and adipose tissue. Also expressed in testis. Detected in a number of tumors including osteosarcoma, mammary carcinoma and hepatocellular carcinoma.
Q5TI25 NBPFE_HUMAN:Expressed in spleen and fetal liver.
Q8N660 NBPFF_HUMAN:Ubiquitously expressed with a higher expression observed in breast and liver. Also expressed in neuroblastoma cell line.
Sequence:
- Q3BBV0 NBPF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVVSAGPWSSEKAETNILEINEKLRPQLAENKQQFRNLKEKCFVTQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHSPCDSNQPHKNINITFEEDKVNSTLVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKLHPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKYEECKDLIKSMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNQHLQALLTPDKPDKSQGQDLQEQLAEGCRLAQQLFQKLSPENDEDEDEDVQVEEAEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPCDSNQPHKNINITFEEDKVNSALVVDRESSHDECQDAVNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKLHPQLAEKKQQFRNLKEKCFVTQLACFLANQQNKYKNEECKDLIKSMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASCSLNQHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQKSSAPREMPKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYKSYSGTFHSLEEQQVCMAVDIGGHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTPSGYLELTDSCQPYRSAFYILEQQRVGWALDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEAVEPEVLQDSLDRCYSTPSSCLEQPDSCLPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSTKKRRRRGRKEGEEDQNPPCPRLSGMLMEVEEPEVLQDSLDRCYSTPSMYFELPDSFQHYRSVFYSFEEQHISFALDVDNRFLTLMGTSLHLVFQMGVIFPQ
- Q5TAG4 NBPFC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVVSAGPWSSEKAEMNILEINEKLRPQLAENKQQFRNLKERCFLTQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQQLVQKLSPENDEDEDEDVQVEEDEKVLESSAPREVQKAEESKVPEDSLEECAITCSNSHGPCDSIQPHKNIKITFEEDKVNSTVVVDRKSSHDECQDALNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKLRPQLAEKKQQFRSLKEKCFVTQLAGFLAKQQNKYKYEECKDLIKSMLRNELQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDEDEDEDVQVEEDEKVLESSSPREMQKAEESKVPEDSLEECAITCSNSHGPCDSNQPHKNIKITFEEDKVNSSLVVDRESSHDECQDALNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKLRPQLAEKKQQFRSLKEKCFVTQVACFLAKQQNKYKYEECKDLLKSMLRNELQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQKSSSPREMQKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPERLASYQSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDQEATGPRLSRELLAEKEPEVLQDSLDRCYSTPSVYLGLTDSCQPYRSAFYVLEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSRELLAEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYRSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKKRRRGRKEGEEDQNPPCPRLSRELLAEKEPEVLQDSLDRWYSTPSVYLGLTDPCQPYRSAFYVLEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSRELLAEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYRSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYRSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKKRRRGRKEGEEDQNPPCPRLNSVLMEVEEPEVLQDSLDRCYSTPSMYFELPDSFQHYRSVFYSFEEQHITFALDMDNSFFTLTVTSLHLVFQMGVIFPQ
- Q5TI25 NBPFE_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRNERQFKEEKLAEQLKQAEELRQYKVLVHAQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVEVAEKVQKSSAPREMQKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYKSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDHEATGPRLSRELLDEKGPEVLQDSLDRCYSTPSGCLELTDSCQPYRSAFYVLEQQRVGLAVNMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLGRCYSTPSGYLELPDLGQPYSSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKERRRGRKEGEEDQNPPCPRLSRELLDEKGPEVLQDSLDRCYSTPSGCLELTDSCQPYRSAFYILEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSGELLDEKEPEVLQESLDRCYSTPSGCLELTDSCQPYRSAFYILEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLGRCYSTPSGYLELPDLGQPYSSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKERRRGRKEGEEDQNPPCPRLNSMLMEVEEPEVLQDSLDICYSTPSMYFELPDSFQHYRSVFYSFEEEHISFALYVDNRFFTLTVTSLHLVFQMGVIFPQ
- Q6P3W6 NBPFA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVVSAGPWSSEKAEMNILEINEKLRPQLAENKQQFGNLKERCFVTQLAGFLANQQKKYNYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHSQERELTQLREKLREGRDASRSLNEHLQALLTLDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDEDEDEDVQVEEAEKVQKSSAPREVQKTEESKVPEDSLEECAITCSNSHGPCDSNQPHKNIKITFEEDEVNSTLVVDRESSHDECQDALNILPVPGPTSSATNVSMVVSAGPLSSEKAEMNILEINEKLHPQLAEKKQQFRNLKERCFVTQLAGFLANQQKKYKYEECKDLIKSMLRNERQFKEEKLAEQLKQAEELRQYKVLVHAQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLAEGCRLAQHLVQKLSPENDNDDDEDVQVELAEKVQKSSAPREMQKAEEKEVPEDSQEECAITYSNSHGPYDSNQPHRKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYQSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDQEATGPRLSRELLDEKGPEVLQDSLDRCYSTPSGCLELTDSCQPYRSAFYVLEQQRVGLAVDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSGYLELPDLGQPYSSAVYSLEEQYLGLALDVDRTKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRS
- Q8N660 NBPFF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVVSAGPLSSEKAEMNILEINEKLRPQLAEKKQQFRNLKEKCFLTQLAGFLANRQKKYKYEECKDLIKFMLRNERQFKEEKLAEQLKQAEELRQYKVLVHAQERELTQLREKLREGRDASRSLNEHLQALLTPDEPDKSQGQDLQEQLAEGCRLTQHLVQKLSPENDNDDDEDVQVEVAEKVQKSSAPREMQKAEEKEVPEDSLEECAITCSNSHGPYDSNQPHKKTKITFEEDKVDSTLIGSSSHVEWEDAVHIIPENESDDEEEEEKGPVSPRNLQESEEEEVPQESWDEGYSTLSIPPEMLASYQSYSSTFHSLEEQQVCMAVDIGRHRWDQVKKEDQEATGPRLSRELLDEKEPEVLQDSLDRCYSTPSGCLELTDSCQPYRSAFYVLEQQRVGLAIDMDEIEKYQEVEEDQDPSCPRLSRELLDEKEPEVLQDSLDRCYSTPSDYLELPDLGQPYSSAVYSLEEQYLGLALDVDRIKKDQEEEEDQGPPCPRLSRELLEVVEPEVLQDSLDRCYSTPSSCLEQPDSCQPYGSSFYALEEKHVGFSLDVGEIEKKGKGKKRRGRRSKKKRRRGRKEGEDDNPPCPRLYGVLMEVEEPEVLQDSLDRCYSTPSMYFEQPDSFQHYRSVFYSFEEEHISFALYVDNRFFTLTVTSLHLVFQMGVIFPQ
PTMs - Q3BBV0/Q5TAG4/Q5TI25/Q6P3W6/Q8N660 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| S961 | Phosphorylation | Uniprot | |
| K973 | Ubiquitination | Uniprot | |
| S981 | Phosphorylation | Uniprot | |
| K1100 | Ubiquitination | Uniprot | |
| K1101 | Acetylation | Uniprot | |
| K1103 | Acetylation | Uniprot | |
| K1105 | Acetylation | Uniprot | |
| K1106 | Acetylation | Uniprot | |
| K1114 | Acetylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K161 | Acetylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K12 | Ubiquitination | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| S669 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| K808 | Ubiquitination | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| T230 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S280 | Phosphorylation | Uniprot | |
| Y331 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| K358 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| K464 | Acetylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| S710 | Phosphorylation | Uniprot | |
| K829 | Ubiquitination | Uniprot | |
| K830 | Acetylation | Uniprot | |
| K832 | Acetylation | Uniprot | |
| K834 | Acetylation | Uniprot | |
| K835 | Acetylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot |
Research Backgrounds
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Widely expressed. The only tissue which shows a weak expression is kidney.
Family&Domains:
Belongs to the NBPF family.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Widely expressed with highest levels in brain, ovary, mammary gland, skin and adipose tissue. Also expressed in testis. Detected in a number of tumors including osteosarcoma, mammary carcinoma and hepatocellular carcinoma.
Family&Domains:
Belongs to the NBPF family.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in spleen and fetal liver.
Family&Domains:
Belongs to the NBPF family.
Subcellular Location:
Cytoplasm.
Family&Domains:
Belongs to the NBPF family.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitously expressed with a higher expression observed in breast and liver. Also expressed in neuroblastoma cell line.
Family&Domains:
Belongs to the NBPF family.
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