NCR3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from A549 cells, using NCR3 antibody.

DF4221 at 1/100 staining mouse liver tissue by IHC-P.

Product:	NCR3 Antibody
Catalog:	DF4221
Description:	Rabbit polyclonal antibody to NCR3
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Mol.Wt.:	30 KD; 22kD(Calculated).
Uniprot:	O14931
RRID:	AB_2836572

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

NCR3 Antibody detects endogenous levels of total NCR3.

RRID:

AB_2836572
Cite Format: Affinity Biosciences Cat# DF4221, RRID:AB_2836572.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1C7; Activating natural killer receptor p30; Activating NK A1 receptor; CD337; CD337 antigen; LY117; Lymphocyte antigen 117; MALS; Natural cytotoxicity triggering receptor 3; Natural killer cell p30 related protein; Natural killer cell p30-related protein; Ncr3; NCTR3_HUMAN; NK-p30; NKp30;

Immunogens

Immunogen:

Uniprot:

O14931(NCTR3_HUMAN) >>Visit HPA database.

Gene(ID):

NCR3(259197)

Expression:

O14931 NCTR3_HUMAN:

Selectively expressed by all resting and activated NK cells and weakly expressed in spleen.

Sequence:

O14931 NCTR3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAWMLLLILIMVHPGSCALWVSQPPEIRTLEGSSAFLPCSFNASQGRLAIGSVTWFRDEVVPGKEVRNGTPEFRGRLAPLASSRFLHDHQAELHIRDVRGHDASIYVCRVEVLGLGVGTGNGTRLVVEKEHPQLGAGTVLLLRAGFYAVSFLSVAVGSTVYYQGKCLTWKGPRRQLPAVVPAPLPPPCGSSAHLLPPVPGG

Research Backgrounds

O14931_NCTR3_HUMAN

Function:

Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Selectively expressed by all resting and activated NK cells and weakly expressed in spleen.

Subunit Structure:

Homodimer in the unliganted form. Interacts with CD3Z. Interacts with and is activated by binding to NCR3LG1. Interacts with and is activated by binding to BAG6.

Family&Domains:

Belongs to the natural cytotoxicity receptor (NCR) family.

Research Fields

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

NCR3 Antibody - #DF4221