CYB5R3 Antibody - #DF4212

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Product: CYB5R3 Antibody
Catalog: DF4212
Description: Rabbit polyclonal antibody to CYB5R3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 34 KD; 34kD(Calculated).
Uniprot: P00387
RRID: AB_2836563

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(90%), Horse(100%), Sheep(90%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
CYB5R3 Antibody detects endogenous levels of total CYB5R3.
RRID:
AB_2836563
Cite Format: Affinity Biosciences Cat# DF4212, RRID:AB_2836563.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B5R; Cyb5r3; Cytochrome b5 reductase 3; Cytochrome b5 reductase; DIA1; Diaphorase 1; Diaphorase-1; NADH cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; NB5R3_HUMAN; OTTHUMP00000028761; OTTHUMP00000198435; OTTHUMP00000198574; OTTHUMP00000198662; OTTHUMP00000198665;

Immunogens

Immunogen:

A synthesized peptide derived from human CYB5R3, corresponding to a region within C-terminal amino acids.

Uniprot:

P00387(NB5R3_HUMAN) >>Visit HPA database.

Gene(ID):
CYB5R3(1727)
Expression:
P00387 NB5R3_HUMAN:

Isoform 2 is expressed at late stages of erythroid maturation.

Sequence:
MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
90
Sheep
90
Dog
90
Rabbit
90
Zebrafish
63
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00387 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
S21 Phosphorylation
T31 Phosphorylation
S38 Phosphorylation
K42 Acetylation
K42 Ubiquitination
Y43 Phosphorylation
S66 Phosphorylation
Y80 Phosphorylation
S82 Phosphorylation
T95 Phosphorylation
K115 Ubiquitination
K120 Ubiquitination
K126 Ubiquitination
S146 Phosphorylation
K154 Ubiquitination
K163 Ubiquitination
K164 Ubiquitination
T171 Phosphorylation
K173 Ubiquitination
S174 Phosphorylation
T182 Phosphorylation
T185 Phosphorylation
K196 Acetylation
K214 Ubiquitination
K234 Ubiquitination
Y248 Phosphorylation

Research Backgrounds

Function:

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Subcellular Location:

Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side. Mitochondrion outer membrane>Lipid-anchor>Cytoplasmic side.

Cytoplasm.
Note: Produces the soluble form found in erythrocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2 is expressed at late stages of erythroid maturation.

Subunit Structure:

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MTARC2.

Family&Domains:

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

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