Product: CYB5R1 Antibody
Catalog: DF4211
Description: Rabbit polyclonal antibody to CYB5R1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 34 KD; 34kD(Calculated).
Uniprot: Q9UHQ9
RRID: AB_2836562

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CYB5R1 Antibody detects endogenous levels of total CYB5R1.
RRID:
AB_2836562
Cite Format: Affinity Biosciences Cat# DF4211, RRID:AB_2836562.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

b5R.1; B5R1; B5R2; CYB5R1; Cytochrome b5 reductase 1; EC 1.6.2.2; Humb5R2; NAD; NAD(P)H:quinone oxidoreductase type 3 polypeptide A2; NAD(P)H:quinone oxidoreductase type 3, polypeptide A2; NADH cytochrome b5 reductase 1; NADH-cytochrome b5 reductase 1; NB5R1_HUMAN; NQO3A2; UNQ3049/PRO9865;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UHQ9 NB5R1_HUMAN:

Widely expressed.

Sequence:
MGIQTSPVLLASLGVGLVTLLGLAVGSYLVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPTAHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKVGDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGPPPMVQLACHPNLDKLGYSQKMRFTY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Horse
91
Zebrafish
90
Bovine
82
Sheep
82
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHQ9 As Substrate

Site PTM Type Enzyme
K54 Ubiquitination
T56 Phosphorylation
K62 Ubiquitination
K81 Ubiquitination
Y84 Phosphorylation
K119 Ubiquitination
K124 Ubiquitination
K130 Methylation
K130 Ubiquitination
K139 Methylation
K139 Ubiquitination
S150 Phosphorylation
K167 Acetylation
K167 Ubiquitination
S169 Phosphorylation
K178 Ubiquitination
T186 Phosphorylation
T189 Phosphorylation
K200 Ubiquitination
K218 Ubiquitination
K248 Ubiquitination
K254 Ubiquitination
T258 Phosphorylation
K300 Ubiquitination

Research Backgrounds

Function:

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

Subcellular Location:

Membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

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