NDUFS3 Antibody - #DF4210

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Product: NDUFS3 Antibody
Catalog: DF4210
Description: Rabbit polyclonal antibody to NDUFS3
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 40 KD; 30kD(Calculated).
Uniprot: O75489
RRID: AB_2836561

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
NDUFS3 Antibody detects endogenous levels of total NDUFS3.
RRID:
AB_2836561
Cite Format: Affinity Biosciences Cat# DF4210, RRID:AB_2836561.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CI 30; CI 30KD; CI-30kD; Complex I 30KD; Complex I 30kDa subunit; COMPLEX I, MITOCHONDRIAL RESPIRATORY CHAIN, 30-KD SUBUNIT; Complex I-30kD; mitochondrial; NADH coenzyme Q reductase; NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa; NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3; NADH dehydrogenase ubiquinone 30 kDa subunit; NADH-ubiquinone oxidoreductase 30 kDa subunit; NADH-Ubiquinone Oxidoreductase Fe-S Protein 3; NDUFS3; NDUS3_HUMAN;

Immunogens

Immunogen:
Uniprot:

O75489(NDUS3_HUMAN) >>Visit HPA database.

Gene(ID):
NDUFS3(4722)
Sequence:
MAAAAVARLWWRGILGASALTRGTGRPSVLLLPVRRESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Horse
92
Xenopus
92
Zebrafish
92
Rabbit
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75489 As Substrate

Site PTM Type Enzyme
S38 Phosphorylation
T46 Phosphorylation
K56 Acetylation
K56 Ubiquitination
S59 Phosphorylation
Y64 Phosphorylation
T115 Phosphorylation
T121 Phosphorylation
K144 Ubiquitination
T151 Phosphorylation
T189 Phosphorylation
Y191 Phosphorylation
K200 Ubiquitination
Y207 Phosphorylation
K232 Ubiquitination
S237 Phosphorylation
S251 Phosphorylation
K253 Ubiquitination
K264 Acetylation

Research Backgrounds

Function:

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).

Subcellular Location:

Mitochondrion inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Mammalian complex I is composed of 45 different subunits. Interacts with NDUFAF3. Interacts with RAB5IF.

Family&Domains:

Belongs to the complex I 30 kDa subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

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For Research Use Only.
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