MINPP1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 cells, using MINPP1 antibody.

Product:	MINPP1 Antibody
Catalog:	DF4189
Description:	Rabbit polyclonal antibody to MINPP1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	60 KD; 55kD(Calculated).
Uniprot:	Q9UNW1
RRID:	AB_2836554

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(82%), Bovine(89%), Horse(100%), Sheep(82%), Rabbit(100%), Dog(91%)

Clonality:

Polyclonal

Specificity:

MINPP1 Antibody detects endogenous levels of total MINPP1.

RRID:

AB_2836554
Cite Format: Affinity Biosciences Cat# DF4189, RRID:AB_2836554.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3; 4; 5)-tetrakisphosphate 3-phosphatase; 5)P(4) 3-phosphatase; DKFZp564L2016; HIPER1; Inositol (1; inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase; Ins(1; ins(1,3,4,5)P(4) 3-phosphatase; MINP1_HUMAN; Minpp1; MINPP2; MIPP; Multiple inositol polyphosphate histidine phosphatase, 1; Multiple inositol polyphosphate phosphatase 1; multiple inositol polyphosphate phosphatase 2;

Immunogens

Immunogen:

Uniprot:

Q9UNW1(MINP1_HUMAN) >>Visit HPA database.

Gene(ID):

MINPP1(9562)

Expression:

Q9UNW1 MINP1_HUMAN:

Widely expressed with highest levels in kidney, liver and placenta.

Sequence:

Q9UNW1 MINP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLRAPGCLLRTSVAPAAALAAALLSSLARCSLLEPRDPVASSLSPYFGTKTRYEDVNPVLLSGPEAPWRDPELLEGTCTPVQLVALIRHGTRYPTVKQIRKLRQLHGLLQARGSRDGGASSTGSRDLGAALADWPLWYADWMDGQLVEKGRQDMRQLALRLASLFPALFSRENYGRLRLITSSKHRCMDSSAAFLQGLWQHYHPGLPPPDVADMEFGPPTVNDKLMRFFDHCEKFLTEVEKNATALYHVEAFKTGPEMQNILKKVAATLQVPVNDLNADLIQVAFFTCSFDLAIKGVKSPWCDVFDIDDAKVLEYLNDLKQYWKRGYGYTINSRSSCTLFQDIFQHLDKAVEQKQRSQPISSPVILQFGHAETLLPLLSLMGYFKDKEPLTAYNYKKQMHRKFRSGLIVPYASNLIFVLYHCENAKTPKEQFRVQMLLNEKVLPLAYSQETVSFYEDLKNHYKDILQSCQTSEECELARANSTSDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Dog

Bovine

Pig

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UNW1 As Substrate

Q9UNW1

Site	PTM Type	Source
T11	Phosphorylation	Uniprot
S42	O-Glycosylation	Uniprot
K50	Ubiquitination	Uniprot
T91	Phosphorylation	Uniprot
N242	N-Glycosylation	Uniprot
K298	Ubiquitination	Uniprot
Y315	Phosphorylation	Uniprot
K463	Ubiquitination	Uniprot

Research Backgrounds

Q9UNW1_MINP1_HUMAN

Function:

Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3-phosphoglycerate. May play a role in bone development (endochondral ossification). May play a role in the transition of chondrocytes from proliferation to hypertrophy (By similarity).

Subcellular Location:

Endoplasmic reticulum lumen.

Tissue Specificity:

Widely expressed with highest levels in kidney, liver and placenta.

Family&Domains:

Belongs to the histidine acid phosphatase family. MINPP1 subfamily.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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MINPP1 Antibody - #DF4189