Product: MRPL4 Antibody
Catalog: DF4176
Description: Rabbit polyclonal antibody to MRPL4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 35 KD; 35kD(Calculated).
Uniprot: Q9BYD3
RRID: AB_2836541

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
MRPL4 Antibody detects endogenous levels of total MRPL4.
RRID:
AB_2836541
Cite Format: Affinity Biosciences Cat# DF4176, RRID:AB_2836541.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39S ribosomal protein L4; 39S ribosomal protein L4 mitochondrial; CDABP0091; CGI-28; L4mt; mitochondrial; Mitochondrial ribosomal protein L4; MNCb-3848; MRP-L4; MRPL4; RM04_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGLNVHSMLKHQTLVLTLPTVAFLEDKLLWQDSRYRPLYPFSLPYSDFPRPLPHATQGPAATPYHC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
90
Zebrafish
80
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BYD3 As Substrate

Site PTM Type Enzyme
S40 Phosphorylation
K50 Ubiquitination
R70 Methylation
K131 Ubiquitination
R147 Methylation
Y161 Phosphorylation
Y162 Phosphorylation
K238 Ubiquitination
Y284 Phosphorylation
S287 Phosphorylation
Y290 Phosphorylation
S291 Phosphorylation

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins. Interacts with MIEF1 upstream open reading frame protein.

Family&Domains:

Belongs to the universal ribosomal protein uL4 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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