Product: MRPS21 Antibody
Catalog: DF4165
Description: Rabbit polyclonal antibody to MRPS21
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 11 KD; 11kD(Calculated).
Uniprot: P82921
RRID: AB_2836530

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
MRPS21 Antibody detects endogenous levels of total MRPS21.
RRID:
AB_2836530
Cite Format: Affinity Biosciences Cat# DF4165, RRID:AB_2836530.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MDS016; mitochondrial 28S ribosomal protein S21; Mitochondrial ribosomal protein S21; MRP S21; S21mt;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAKHLKFIARTVMVQEGNVESAYRTLNRILTMDGLIEDIKHRRYYEKPCCRRQRESYERCRRIYNMEMARKINFLMRKNRADPWQGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
89
Xenopus
89
Chicken
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P82921 As Substrate

Site PTM Type Enzyme
T31 Phosphorylation
K40 Ubiquitination
Y64 Phosphorylation
C87 S-Nitrosylation

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Family&Domains:

Belongs to the bacterial ribosomal protein bS21 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

References

1). C17orf80 binds the mitochondrial genome to promote its replication. Journal of Cell Biology, 2023 (PubMed: 37676315) [IF=7.8]

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