Product: MGST1 Antibody
Catalog: DF4161
Description: Rabbit polyclonal antibody to MGST1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 18 KD; 18kD(Calculated).
Uniprot: P10620
RRID: AB_2836526

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(82%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MGST1 Antibody detects endogenous levels of total MGST1.
RRID:
AB_2836526
Cite Format: Affinity Biosciences Cat# DF4161, RRID:AB_2836526.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGST1; Glutathione S transferase 12; GST12; MGST 1; MGST; MGST I; MGST1; MGST1_HUMAN; Microsomal glutathione S-transferase 1; Microsomal GST 1; Microsomal GST-1; Microsomal GST-I;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P10620 MGST1_HUMAN:

Highly expressed in liver.

Sequence:
MVDLTQVMDDEVFMAFASYATIILSKMMLMSTATAFYRLTRKVFANPEDCVAFGKGENAKKYLRTDDRVERVRRAHLNDLENIIPFLGIGLLYSLSGPDPSTAILHFRLFVGARIYHTIAYLTPLPQPNRALSFFVGYGVTLSMAYRLLKSKLYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
91
Rabbit
82
Xenopus
70
Chicken
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10620 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation
Y37 Phosphorylation
T40 Phosphorylation
K42 Acetylation
C50 S-Nitrosylation
K55 Acetylation
K55 Ubiquitination
K60 Ubiquitination
Y121 Phosphorylation
T123 Phosphorylation

Research Backgrounds

Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

PTMs:

Peroxynitrite induces nitration at Tyr-93 which activates the enzyme.

Subcellular Location:

Microsome. Mitochondrion outer membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in liver.

Subunit Structure:

Homotrimer; The trimer binds only one molecule of glutathione.

Family&Domains:

Belongs to the MAPEG family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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