Product: MBTPS1 Antibody
Catalog: DF4159
Description: Rabbit polyclonal antibody to MBTPS1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 117 KD; 118kD(Calculated).
Uniprot: Q14703
RRID: AB_2836524

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MBTPS1 Antibody detects endogenous levels of total MBTPS1.
RRID:
AB_2836524
Cite Format: Affinity Biosciences Cat# DF4159, RRID:AB_2836524.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

kexin isozyme 1; KIAA0091; MBTPS1; membrane bound transcription factor peptidase, site 1; MGC138711; MGC138712; PCSK8; S1P; S1P endopeptidase; Site 1 protease; SKI 1; SKI1; Subtilisin isozyme 1; Subtilisin/kexin isozyme 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q14703 MBTP1_HUMAN:

Widely expressed.

Sequence:
MKLVNIWLLLLVVLLCGKKHLGDRLEKKSFEKAPCPGCSHLTLKVEFSSTVVEYEYIVAFNGYFTAKARNSFISSALKSSEVDNWRIIPRNNPSSDYPSDFEVIQIKEKQKAGLLTLEDHPNIKRVTPQRKVFRSLKYAESDPTVPCNETRWSQKWQSSRPLRRASLSLGSGFWHATGRHSSRRLLRAIPRQVAQTLQADVLWQMGYTGANVRVAVFDTGLSEKHPHFKNVKERTNWTNERTLDDGLGHGTFVAGVIASMRECQGFAPDAELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANNVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRMKPDIVTYGAGVRGSGVKGGCRALSGTSVASPVVAGAVTLLVSTVQKRELVNPASMKQALIASARRLPGVNMFEQGHGKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTVVNVTILNGMGVTGRIVDKPDWQPYLPQNGDNIEVAFSYSSVLWPWSGYLAISISVTKKAASWEGIAQGHVMITVASPAETESKNGAEQTSTVKLPIKVKIIPTPPRSKRVLWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPFTCFDASQYGTLLMVDSEEEYFPEEIAKLRRDVDNGLSLVIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANIPALNELLSVWNMGFSDGLYEGEFTLANHDMYYASGCSIAKFPEDGVVITQTFKDQGLEVLKQETAVVENVPILGLYQIPAEGGGRIVLYGDSNCLDDSHRQKDCFWLLDALLQYTSYGVTPPSLSHSGNRQRPPSGAGSVTPERMEGNHLHRYSKVLEAHLGDPKPRPLPACPRLSWAKPQPLNETAPSNLWKHQKLLSIDLDKVVLPNFRSNRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPVFAFLGAMVVLAFFVVQINKAKSRPKRRKPRVKRPQLMQQVHPPKTPSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14703 As Substrate

Site PTM Type Enzyme
K78 Ubiquitination
K107 Ubiquitination
K137 Ubiquitination
S166 O-Glycosylation
S168 O-Glycosylation
S168 Phosphorylation
T177 O-Glycosylation
T177 Phosphorylation
R213 Methylation
K224 Ubiquitination
K229 Ubiquitination
T235 Phosphorylation
K388 Ubiquitination
S417 Phosphorylation
S429 Phosphorylation
K443 Ubiquitination
T616 Phosphorylation
K646 Ubiquitination
T896 O-Glycosylation
K934 Ubiquitination
K951 Ubiquitination
K1036 Acetylation
K1048 Acetylation
T1049 Phosphorylation
S1051 Phosphorylation

Research Backgrounds

Function:

Serine protease that catalyzes the first step in the proteolytic activation of the sterol regulatory element-binding proteins (SREBPs). Other known substrates are BDNF, GNPTAB and ATF6. Cleaves after hydrophobic or small residues, provided that Arg or Lys is in position P4. Cleaves known substrates after Arg-Ser-Val-Leu (SERBP-2), Arg-His-Leu-Leu (ATF6), Arg-Gly-Leu-Thr (BDNF) and its own propeptide after Arg-Arg-Leu-Leu. Mediates the protein cleavage of GNPTAB into subunit alpha and beta, thereby participating in biogenesis of lysosomes. Involved in the regulation of M6P-dependent Golgi-to-lysosome trafficking of lysosomal enzymes. It is required for the activation of CREB3L2/BBF2H7, a transcriptional activator of MIA3/TANGO and other genes controlling mega vesicle formation. Therefore, it plays a key role in the regulation of mega vesicle-mediated collagen trafficking.

PTMs:

The 148 kDa zymogen is processed progressively into two membrane-bound 120 and 106 kDa forms in the endoplasmic reticulum, and late into a secreted 98 kDa form. The propeptide is autocatalytically removed through an intramolecular cleavage after Leu-186. Further cleavage generates 14, 10, and 8 kDa intermediates.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type I membrane protein. Golgi apparatus membrane>Single-pass type I membrane protein.
Note: May sort to other organelles, including lysosomal and/or endosomal compartments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Family&Domains:

Belongs to the peptidase S8 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

References

1). Hydrogen sulfide alleviates ischemia induced liver injury by repressing the SPHK1/S1P pathway. Annals of Translational Medicine, 2023 (PubMed: 36819566)

Application: WB    Species: Rat    Sample: liver

Figure 1 NaHS decreases endoplasmic reticulum stress and hepatic cell viability under hypoxic conditions. (A) MTT analysis of BRL-3A cell viability after sham, hypoxia, and NaHS treatment. (B) Hoechst staining analysis of BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×200). (C) Left: representative Western blot analysis of the protein expression of CHOP, SPHK1, and S1P. Right: corresponding quantification of the band densities of CHOP, SPHK1, and S1P. (D) Immunofluorescence analysis of CHOP and SPHK1 levels in BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×400). **, P

Application: WB    Species: Rat    Sample: liver

Figure 1 NaHS decreases endoplasmic reticulum stress and hepatic cell viability under hypoxic conditions. (A) MTT analysis of BRL-3A cell viability after sham, hypoxia, and NaHS treatment. (B) Hoechst staining analysis of BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×200). (C) Left: representative Western blot analysis of the protein expression of CHOP, SPHK1, and S1P. Right: corresponding quantification of the band densities of CHOP, SPHK1, and S1P. (D) Immunofluorescence analysis of CHOP and SPHK1 levels in BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×400). **, P

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