REC8 Antibody - #DF4155

(1)  
Product: REC8 Antibody
Catalog: DF4155
Description: Rabbit polyclonal antibody to REC8
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 62 KD; 63kD(Calculated).
Uniprot: O95072
RRID: AB_2836520

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(91%), Bovine(82%), Sheep(82%)
Clonality:
Polyclonal
Specificity:
REC8 Antibody detects endogenous levels of total REC8.
RRID:
AB_2836520
Cite Format: Affinity Biosciences Cat# DF4155, RRID:AB_2836520.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cohesin Rec8p; Human homolog of rad21 S. pombe; Meiotic recombination and sister chromatid cohesion phosphoprotein of the rad21p family; Meiotic recombination protein REC8 homolog; Meiotic recombination protein REC8-like 1; rec8; REC8 homolog (yeast); REC8 like 1; REC8_HUMAN; Rec8p; Recombination and sister chromatid cohesion protein homolog;

Immunogens

Immunogen:
Uniprot:

O95072(REC8_HUMAN) >>Visit HPA database.

Gene(ID):
REC8(9985)
Expression:
O95072 REC8_HUMAN:

Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level).

Sequence:
MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLRVNVVKTCEEILNYVLVRVQPPQPGLPRPRFSLYLSAQLQIGVIRVYSQQCQYLVEDIQHILERLHRAQLQIRIDMETELPSLLLPNHLAMMETLEDAPDPFFGMMSVDPRLPSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
82
Sheep
82
Rabbit
73
Dog
64
Xenopus
45
Horse
36
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95072 As Substrate

Site PTM Type Enzyme
T17 Phosphorylation
T23 Phosphorylation
S148 Phosphorylation
T176 Phosphorylation
S286 Phosphorylation
S398 Phosphorylation
S410 Phosphorylation
S499 Phosphorylation

Research Backgrounds

Function:

Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).

PTMs:

Phosphorylated.

Subcellular Location:

Nucleus. Chromosome. Chromosome>Centromere.
Note: In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II. Localised to centromeres and spindle poles in endopolyploid tumor cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level).

Subunit Structure:

Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51 (By similarity). Forms a complex with EWSR1, PRDM9, SYCP3 and SYCP1; complex formation is dependent of phosphorylated form of REC8 and requires PRDM9 bound to hotspot DNA; EWSR1 joins PRDM9 with the chromosomal axis through REC8 (By similarity).

Family&Domains:

Belongs to the rad21 family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

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