MRC1/CD206 Antibody - #DF4149

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).B, Western blot analysis of IL-33 and CD206 expression in non-tumour and tumour tissues, and GAPDH was used as a reference control.

Fig. 6 Inhibition of TRPA1 diminishes macrophage polarization to the M2 phenotype by disrupting the phosphorylation of the TGF-β1-suppressor of Smad2 pathway. A Western blot analysis of TGF-β1-Smad2 pathway activation and the expression of macrophage polarization markers CD206, IL-4, IL-10, and IL-13. B Immunohistochemical staining to detect the expression of CD206, IL-4, IL-10, and IL-13 proteins. C Schematic representation of cellular localization and quantification of CD206, IL-4, IL-10, and IL-13. Statistical significance was denoted as *P (Bleomycin group vs. Control group, *P 

Fig. 6 Inhibition of TRPA1 diminishes macrophage polarization to the M2 phenotype by disrupting the phosphorylation of the TGF-β1-suppressor of Smad2 pathway. A Western blot analysis of TGF-β1-Smad2 pathway activation and the expression of macrophage polarization markers CD206, IL-4, IL-10, and IL-13. B Immunohistochemical staining to detect the expression of CD206, IL-4, IL-10, and IL-13 proteins. C Schematic representation of cellular localization and quantification of CD206, IL-4, IL-10, and IL-13. Statistical significance was denoted as *P (Bleomycin group vs. Control group, *P 

Fig. 3. HXP reduced neuroinflammation in MCAO rats by promoting MCPIP1-mediated microglia M2 polarization. A. After reperfusion for 7 d, brain water contents were examined. B. Brain infract volume was detected by 2,3,5-Triphentltetrazolium chloride staining. C. Changes in IL-1β, IL-6, iNOS, and TNF-α were measured via ELISA assy. D. Representative images of histopathological analysis. Scale bar, 20 µm. E. Representative immunofluorescence staining for CD206 (red) and Iba1 (green) in brain tissues. Scale bar, 20 µm. F. The expression of MCPIP1, CD16, iNOS, CD206, Arg1, and PPARγ was detected using Western blotting assay. Data are represented as mean ± standard deviation (N = 5). * * P 

Fig. 3. HXP reduced neuroinflammation in MCAO rats by promoting MCPIP1-mediated microglia M2 polarization. A. After reperfusion for 7 d, brain water contents were examined. B. Brain infract volume was detected by 2,3,5-Triphentltetrazolium chloride staining. C. Changes in IL-1β, IL-6, iNOS, and TNF-α were measured via ELISA assy. D. Representative images of histopathological analysis. Scale bar, 20 µm. E. Representative immunofluorescence staining for CD206 (red) and Iba1 (green) in brain tissues. Scale bar, 20 µm. F. The expression of MCPIP1, CD16, iNOS, CD206, Arg1, and PPARγ was detected using Western blotting assay. Data are represented as mean ± standard deviation (N = 5). * * P 

Fig. 7. TREM2 promoted phagocytic activity of Mi/MΦ and attenuated neutrophil infiltration at 48 h after SAH. A and C, Phagocytic Mi/MΦ identified by CD206 (red) and CD11b (yellow) co-staining were increased in the basal cortex of temporal lobe at 48 h after SAH. TREM2 overexpression up-regulated the differentiation of CD206+/CD11b+ Mi/MΦ, while TREM2 knockdown down-regulated these phagocytic Mi/MΦ. CD206+/CD11b+ Mi/MΦ (C), $P < 0.001 in SAH vs Sham, @P = 0.03 in SAH + Lv-Trem2 vs. SAH + Lv-con, &P = 0.002 in SAH + shRNA-Trem2 vs. SAH + shRNA-con; ns, P > 0.9 in SAH + Lv-con and P > 0.9 in SAH + shRNA-con vs. SAH. B and D, IF staining showed significant neutrophil infiltration in the basal cortex at 48 h after SAH, manifested as more MPO (red) staining cells. TREM2 knockdown further increased the numbers of infiltrated MPO+ cells, while TREM2 overexpression significantly decreased the numbers of infiltrated MPO+ cells. MPO+ cells (D), $P < 0.001 in SAH vs Sham, @P < 0.001 in SAH + Lv-Trem2 vs. SAH + Lv-con, &P < 0.001 in SAH + shRNA-Trem2 vs. SAH + shRNA-con; ns, P > 0.9 in SAH + Lv-con and P > 0.9 in SAH + shRNA-con vs. SAH. Scale bar = 50 μm, n = 6 per group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Figure 4 PARP14 deficiency exacerbates the shift of M2-polarized microglia to M1-polarized microglia in mice 7 days post-SCI. (A) Representative images and quantitative analysis showing iNOS+/Iba1+ immunofluorescence staining. Lv-shPARP14 injection enhanced the SCI-induced increase in iNOS expression (pro-inflammatory phenotype). White arrows indicate iNOS+ (green, FITC-labeled)/Iba1+ (red, Cy3-labeled, microglia marker) cells. (B) Representative images and quantitative analysis showing Arg-1+/Iba1+ immunofluorescence staining. Lv-shPARP14 injection reversed the SCI-induced increase in Arg-1 expression (anti-inflammatory phenotype). White arrows indicate Arg-1+ (green, FITC-labeled)/Iba1+ (red, Cy3-labeled, microglia marker) cells. Scale bars: 50 µm in A and B. (C) Relative protein expression of CD16 and CD206 in each group. Lv-shPARP14 injection enhanced the SCI-induced increase in CD16 (M1-type marker) expression and decreased the SCI-induced increase in CD206 (M2-type marker) expression. (D) The concentration of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and anti-inflammatory cytokines (IL-10, TGF-β1, and IL-4) in each group was measured by enzyme-linked immunosorbent assay. Lv-shPARP14 injection increased the concentration of pro-inflammatory cytokines but decreased anti-inflammatory cytokine accumulation. Values are shown as mean ± SD (n = 6). *P < 0.05, **P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). Images were taken of the gray matter ventral horn at the injury site. Spinal cord tissues from the injury site were used for western blot and enzyme-linked immunosorbent assay detection. Arg-1: Arginase-1; DAPI: 4′,6-diamidino-2-phenylindole; FITC: fluorescein isothiocyanate; Iba1: ionized calcium-binding adaptor molecule 1; IL: interleukin; iNOS: inducible nitric oxide synthase; PARP14: poly(ADP-ribose)polymerase, member 14; SCI: spinal cord injury; TGF-β1: transforming growth factor-beta1; TNF-α: tumor necrosis factor alpha.