Product: MRC1 Antibody
Catalog: DF4149
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 166 kD; 166kD(Calculated).
Uniprot: P22897
RRID: AB_2836514

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:100, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
MRC1 Antibody detects endogenous levels of total MRC1.
RRID:
AB_2836514
Cite Format: Affinity Biosciences Cat# DF4149, RRID:AB_2836514.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA541I19.1; C type lectin domain family 13 member D; C-type lectin domain family 13 member D; CD 206; CD206; CD206 antigen; CLEC13D; CLEC13DL; Macrophage mannose receptor 1; Macrophage mannose receptor 1 like protein 1; Macrophage mannose receptor; Mannose receptor C type 1; Mannose receptor C type 1 like 1; MMR; MRC 1; MRC1; MRC1_HUMAN; MRC1L1; OTTHUMP00000045206;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MRLPLLLVFASVIPGAVLLLDTRQFLIYNEDHKRCVDAVSPSAVQTAACNQDAESQKFRWVSESQIMSVAFKLCLGVPSKTDWVAITLYACDSKSEFQKWECKNDTLLGIKGEDLFFNYGNRQEKNIMLYKGSGLWSRWKIYGTTDNLCSRGYEAMYTLLGNANGATCAFPFKFENKWYADCTSAGRSDGWLWCGTTTDYDTDKLFGYCPLKFEGSESLWNKDPLTSVSYQINSKSALTWHQARKSCQQQNAELLSITEIHEQTYLTGLTSSLTSGLWIGLNSLSFNSGWQWSDRSPFRYLNWLPGSPSAEPGKSCVSLNPGKNAKWENLECVQKLGYICKKGNTTLNSFVIPSESDVPTHCPSQWWPYAGHCYKIHRDEKKIQRDALTTCRKEGGDLTSIHTIEELDFIISQLGYEPNDELWIGLNDIKIQMYFEWSDGTPVTFTKWLRGEPSHENNRQEDCVVMKGKDGYWADRGCEWPLGYICKMKSRSQGPEIVEVEKGCRKGWKKHHFYCYMIGHTLSTFAEANQTCNNENAYLTTIEDRYEQAFLTSFVGLRPEKYFWTGLSDIQTKGTFQWTIEEEVRFTHWNSDMPGRKPGCVAMRTGIAGGLWDVLKCDEKAKFVCKHWAEGVTHPPKPTTTPEPKCPEDWGASSRTSLCFKLYAKGKHEKKTWFESRDFCRALGGDLASINNKEEQQTIWRLITASGSYHKLFWLGLTYGSPSEGFTWSDGSPVSYENWAYGEPNNYQNVEYCGELKGDPTMSWNDINCEHLNNWICQIQKGQTPKPEPTPAPQDNPPVTEDGWVIYKDYQYYFSKEKETMDNARAFCKRNFGDLVSIQSESEKKFLWKYVNRNDAQSAYFIGLLISLDKKFAWMDGSKVDYVSWATGEPNFANEDENCVTMYSNSGFWNDINCGYPNAFICQRHNSSINATTVMPTMPSVPSGCKEGWNFYSNKCFKIFGFMEEERKNWQEARKACIGFGGNLVSIQNEKEQAFLTYHMKDSTFSAWTGLNDVNSEHTFLWTDGRGVHYTNWGKGYPGGRRSSLSYEDADCVVIIGGASNEAGKWMDDTCDSKRGYICQTRSDPSLTNPPATIQTDGFVKYGKSSYSLMRQKFQWHEAETYCKLHNSLIASILDPYSNAFAWLQMETSNERVWIALNSNLTDNQYTWTDKWRVRYTNWAADEPKLKSACVYLDLDGYWKTAHCNESFYFLCKRSDEIPATEPPQLPGRCPESDHTAWIPFHGHCYYIESSYTRNWGQASLECLRMGSSLVSIESAAESSFLSYRVEPLKSKTNFWIGLFRNVEGTWLWINNSPVSFVNWNTGDPSGERNDCVALHASSGFWSNIHCSSYKGYICKRPKIIDAKPTHELLTTKADTRKMDPSKPSSNVAGVVIIVILLILTGAGLAAYFFYKKRRVHLPQEGAFENTLYFNSQSSPGTSDMKDLVGNIEQNEHSVI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22897 As Substrate

Site PTM Type Enzyme
S62 Phosphorylation
S64 Phosphorylation
Y200 Phosphorylation
T389 Phosphorylation
T390 Phosphorylation
T633 Phosphorylation
K670 Acetylation
T820 Phosphorylation
Y850 Phosphorylation
Y860 Phosphorylation
T1081 Phosphorylation
S1128 Phosphorylation
N1205 N-Glycosylation
S1268 Phosphorylation
S1279 Phosphorylation
S1280 Phosphorylation
S1454 Phosphorylation

Research Backgrounds

Function:

Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains.

(Microbial infection) Acts as phagocytic receptor for bacteria, fungi and other pathogens.

(Microbial infection) Acts as a receptor for Dengue virus envelope protein E.

(Microbial infection) Interacts with Hepatitis B virus envelope protein.

Subcellular Location:

Endosome membrane>Single-pass type I membrane protein. Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

(Microbial infection) Interacts with Dengue virus.

(Microbial infection) May act as a receptor for hepatitis B virus, enabling uptake of the virus in hepatic dendritic cells.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

References

1). Zhang G et al. Soft apoptotic-cell-inspired nanoparticles persistently bind to macrophage membranes and promote anti-inflammatory and pro-healing effects. Acta Biomater 2021 Sep 1;131:452-463. (PubMed: 34245890) [IF=10.633]

2). Mai S et al. Oesophageal squamous cell carcinoma–associated IL‐33 rewires macrophage polarization towards M2 via activating ornithine decarboxylase. Cell Prolif 2020 Dec 10;e12960. (PubMed: 33305406) [IF=8.755]

Application: IHC    Species: human    Sample: non-tumour and tumour

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).

Application: WB    Species: human    Sample: non-tumour and tumour

FIGURE 1|M2 macrophage infiltration and IL-33 production are enhanced with close correlation in oesophageal squamous cell carcinoma (ESCC). A, Representative images of IL-33+ cell, CD206+ cell and CD68+ cell in non-tumour and tumour tissue (scale bar = 50 μm).B, Western blot analysis of IL-33 and CD206 expression in non-tumour and tumour tissues, and GAPDH was used as a reference control.

3). Wang M et al. Atractylenolide III Attenuates Muscle Wasting in Chronic Kidney Disease via the Oxidative Stress-Mediated PI3K/AKT/mTOR Pathway. Oxid Med Cell Longev 2019 Apr 18;2019:1875471 (PubMed: 31178951) [IF=7.310]

Application: IF/ICC    Species: rat    Sample: CD68+ macrophages

FIGURE S1.| Macrophage markers immunofluorescence in skeletal muscles within the sham group, CKD group and ATL-III treatment group.(a-b) Representative immunofluorescence images demonstrating CD68+ macrophages,CD68+ / CD86+ macrophages (M1), and CD68+ /CD206+ macrophages (M2) in muscle biopsies of sham group, CKD group and ATL-III treatment group.

4). Li S et al. Clevudine attenuates bleomycin-induced early pulmonary fibrosis via regulating M2 macrophage polarization. Int Immunopharmacol 2021 Oct 23;101(Pt B):108271. (PubMed: 34700113) [IF=5.714]

5). Qiao G et al. Isosteviol reduces the acute inflammatory response after burns by upregulating MMP9 in macrophages leading to M2 polarization. Int Immunopharmacol 2022 Feb 14;106:108609. (PubMed: 35176589) [IF=5.714]

6). Li X et al. Zanubrutinib ameliorates lipopolysaccharide-induced acute lung injury via regulating macrophage polarization. Int Immunopharmacol 2022 Aug 13;111:109138. (PubMed: 35973369) [IF=5.714]

7). Luo ZW et al. Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation. World J Stem Cells 2021 Nov 26;13(11):1762-1782. (PubMed: 34909122) [IF=5.247]

8). Luo Z et al. Bone Marrow Stromal Cell-Derived Exosomes promote muscle healing following contusion through macrophage polarization. Stem Cells Dev 2020 Dec 15. (PubMed: 33323007) [IF=4.390]

Application: WB    Species: Mice    Sample: tibialis anterior muscles

Figure 7. BMSC-Exos induced M2 macrophage polarization in vivo. (A-F) iNOS, CD86, CD206, and Arg1 protein levels in tibialis anterior muscles were determined by western blot at day 3 (n=4). Data are expressed as the means ± standard error of the mean. * P<0.05, ** P<0.01. (G) Immunofluorescence localization and relative expression of CD206, maker of M2, in tibialis anterior muscles between contusion group and BMSC-Exos group (contusion + BMSC-Exos injection) at day 3. All photographs were taken at 200x magnification. Red arrows pointed to CD206 positive cells.

Application: IF/ICC    Species: Mice    Sample: tibialis anterior muscles

Figure 7. BMSC-Exos induced M2 macrophage polarization in vivo. (A-F) iNOS, CD86, CD206, and Arg1 protein levels in tibialis anterior muscles were determined by western blot at day 3 (n=4). Data are expressed as the means ± standard error of the mean. * P<0.05, ** P<0.01. (G) Immunofluorescence localization and relative expression of CD206, maker of M2, in tibialis anterior muscles between contusion group and BMSC-Exos group (contusion + BMSC-Exos injection) at day 3. All photographs were taken at 200x magnification. Red arrows pointed to CD206 positive cells.

9). He B et al. Fluorescence visualization of the neuropathic pain triad in trigeminal neuralgia. J Biophotonics 2022 Nov 12;e202200301. (PubMed: 36369929) [IF=3.390]

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