Product: LIPI Antibody
Catalog: DF4141
Description: Rabbit polyclonal antibody to LIPI
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 53 KD; 53kD(Calculated).
Uniprot: Q6XZB0
RRID: AB_2836506

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
LIPI Antibody detects endogenous levels of total LIPI.
RRID:
AB_2836506
Cite Format: Affinity Biosciences Cat# DF4141, RRID:AB_2836506.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cancer/testis antigen 17; CT17; Lipase member I; LIPI; LIPI_HUMAN; LPD lipase; LPDL; Membrane-associated phosphatidic acid-selective phospholipase A1-beta; membrane-associated phospholipase A1 beta 7B+; membrane-associated phospholipase A1 beta deltaE2-3; membrane-associated phospholipase A1 beta deltaE5; membrane-associated phospholipase A1 beta deltaE6.1; membrane-associated phospholipase A1 beta deltaE7.2; membrane-associated phospholipase A1 beta deltaE8-9; membrane-associated phospholipase A1 beta fl; mPA-PLA1 beta; PLA1C; PRED5;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q6XZB0 LIPI_HUMAN:

Expressed in testis. Expressed exclusively at the connecting piece of the sperm.

Sequence:
MRVYIFLCLMCWVRSDNKRPCLEFSQLSVKDSFRDLFIPRIETILMMYTRNNLNCAEPLFEQNNSLNVNFNTQKKTVWLIHGYRPVGSIPLWLQNFVRILLNEEDMNVIVVDWSRGATTFIYNRAVKNTRKVAVSLSVHIKNLLKHGASLDNFHFIGVSLGAHISGFVGKIFHGQLGRITGLDPAGPRFSRKPPYSRLDYTDAKFVDVIHSDSNGLGIQEPLGHIDFYPNGGNKQPGCPKSIFSGIQFIKCNHQRAVHLFMASLETNCNFISFPCRSYKDYKTSLCVDCDCFKEKSCPRLGYQAKLFKGVLKERMEGRPLRTTVFLDTSGTYPFCTYYFVLSIIVPDKTMMDGSFSFKLLNQLGMIEEPRLYEKNKPFYKLQEVKILAQFYNDFVNISSIGLTYFQSSNLQCSTCTYKIQSLMLKSLTYPERPPLCRYNIVLKDREEVFLNPNTCTPKNT

PTMs - Q6XZB0 As Substrate

Site PTM Type Enzyme
S25 Phosphorylation
T43 Phosphorylation
Y48 Phosphorylation
T49 Phosphorylation
S135 Phosphorylation

Research Backgrounds

Function:

Hydrolyzes specifically phosphatidic acid (PA) to produce 2-acyl lysophosphatidic acid (LPA; a potent bioactive lipid mediator) and fatty acid. Does not hydrolyze other phospholipids, like phosphatidylserine (PS), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) or triacylglycerol (TG).

Subcellular Location:

Membrane. Secreted.
Note: May associate with lipid draft.

Membrane. Secreted.
Note: May associate with lipid draft.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in testis. Expressed exclusively at the connecting piece of the sperm.

Subunit Structure:

Interacts with heparin with a high affinity.

Family&Domains:

Belongs to the AB hydrolase superfamily. Lipase family.

Restrictive clause

 

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