KCNN4 Antibody - #DF4132

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Product: KCNN4 Antibody
Catalog: DF4132
Description: Rabbit polyclonal antibody to KCNN4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 48 KD; 48kD(Calculated).
Uniprot: O15554
RRID: AB_2836497

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KCNN4 Antibody detects endogenous levels of total KCNN4.
RRID:
AB_2836497
Cite Format: Affinity Biosciences Cat# DF4132, RRID:AB_2836497.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHS2; hIKCa1; hKCa4; hSK4; IK; IK1; IKCa1; Intermediate conductance calcium activated potassium channel protein 4; Intermediate conductance calcium-activated potassium channel protein 4; KCa3.1; KCa4; KCNN 4; Kcnn4; KCNN4_HUMAN; Potassium calcium activated channel subfamily N member 4; Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4; Putative Gardos channel; SK4; SKCa 4; SKCa4;

Immunogens

Immunogen:
Uniprot:

O15554(KCNN4_HUMAN) >>Visit HPA database.

Gene(ID):
KCNN4(3783)
Expression:
O15554 KCNN4_HUMAN:

Widely expressed in non-excitable tissues.

Sequence:
MGGDLVLGLGALRRRKRLLEQEKSLAGWALVLAGTGIGLMVLHAEMLWFGGCSWALYLFLVKCTISISTFLLLCLIVAFHAKEVQLFMTDNGLRDWRVALTGRQAAQIVLELVVCGLHPAPVRGPPCVQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Rabbit
100
Horse
91
Zebrafish
67
Bovine
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15554 As Substrate

Site PTM Type Enzyme
S178 Phosphorylation
Y199 Phosphorylation
S314 Phosphorylation
T329 Phosphorylation
S334 Phosphorylation P17612 (PRKACA)
H358 Phosphorylation P22392 (NME2)
S367 Phosphorylation
K394 Ubiquitination
T398 Phosphorylation

Research Backgrounds

Function:

Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis.

PTMs:

Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in non-excitable tissues.

Subunit Structure:

Heterotetramer of potassium channel proteins (Probable). Interacts with MTMR6.

Family&Domains:

Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.

Research Fields

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Protein digestion and absorption.

References

1). A prospective diagnostic and prognostic biomarker for hepatocellular carcinoma that functions in glucose metabolism regulation: Solute carrier family 37 member 3. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (PubMed: 36773462) [IF=6.2]
2). KCNN4 is a potential prognostic marker and critical factor affecting the immune status of the tumor microenvironment in kidney renal clear cell carcinoma. Translational Andrology and Urology (PubMed: 34295732) [IF=2.0]

Application: WB    Species: Human    Sample: kidney renal clear cell

Figure 13 Expression of KCNN4 increased in KIRC samples. (A) Expression of KCNN4 in KIRC samples and normal adjacent non-tumor controls were assessed by qPCR (*P<0.05). (B,C) Representative blots and quantification results of western blot showed that expression of the KCNN4 protein in KIRC was significantly higher than controls (*P<0.05). KIRC, kidney renal clear cell carcinoma.

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