ICAM2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from NIH-3T3 cells, using ICAM2 antibody.

Product:	ICAM2 Antibody
Catalog:	DF4131
Description:	Rabbit polyclonal antibody to ICAM2
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	30 KD; 31kD(Calculated).
Uniprot:	P13598
RRID:	AB_2836496

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

ICAM2 Antibody detects endogenous levels of total ICAM2.

RRID:

AB_2836496
Cite Format: Affinity Biosciences Cat# DF4131, RRID:AB_2836496.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD102; CD102 antigen; ICAM 2; Intercellular adhesion molecule 2; Ly60;

Immunogens

Immunogen:

Uniprot:

P13598(ICAM2_HUMAN) >>Visit HPA database.

Gene(ID):

ICAM2(3384)

Sequence:

P13598 ICAM2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSFGYRTLTVALFTLICCPGSDEKVFEVHVRPKKLAVEPKGSLEVNCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNETLHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSAPKMLEIYEPVSDSQMVIIVTVVSVLLSLFVTSVLLCFIFGQHLRQQRMGTYGVRAAWRRLPQAFRP

PTMs - P13598 As Substrate

P13598

Site	PTM Type	Source
N47	N-Glycosylation	Uniprot
N82	N-Glycosylation	Uniprot
N105	N-Glycosylation	Uniprot
N153	N-Glycosylation	Uniprot
K163	Ubiquitination	Uniprot
N176	N-Glycosylation	Uniprot
N187	N-Glycosylation	Uniprot
K206	Ubiquitination	Uniprot
T259	Phosphorylation	Uniprot
Y260	Phosphorylation	Uniprot

Research Backgrounds

P13598_ICAM2_HUMAN

Function:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Cell projection>Microvillus.
Note: Co-localizes with RDX, EZR and MSN in microvilli.

Subunit Structure:

Interacts with RDX, EZR and MSN.

Family&Domains:

Belongs to the immunoglobulin superfamily. ICAM family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ICAM2 Antibody - #DF4131