SIX1 Antibody - #DF4129

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Product: SIX1 Antibody
Catalog: DF4129
Description: Rabbit polyclonal antibody to SIX1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 33 KD; 32kD(Calculated).
Uniprot: Q15475
RRID: AB_2836494

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SIX1 Antibody detects endogenous levels of total SIX1.
RRID:
AB_2836494
Cite Format: Affinity Biosciences Cat# DF4129, RRID:AB_2836494.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BOS3; DFNA23; Homeobox protein SIX1; OTTHUMP00000179042; Sine oculis homeobox homolog 1; SIX homeobox 1; SIX1; SIX1_HUMAN; TIP39;

Immunogens

Immunogen:
Uniprot:

Q15475(SIX1_HUMAN) >>Visit HPA database.

Gene(ID):
SIX1(6495)
Expression:
Q15475 SIX1_HUMAN:

Specifically expressed in skeletal muscle.

Sequence:
MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYVEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDSLLGPLTSSLVDLGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15475 As Substrate

Site PTM Type Enzyme
K53 Ubiquitination
K97 Ubiquitination
Y143 Phosphorylation
S150 Phosphorylation
S204 Phosphorylation

Research Backgrounds

Function:

Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1.

PTMs:

Phosphorylated during interphase; becomes hyperphosphorylated during mitosis. Hyperphosphorylation impairs binding to promoter elements.

Ubiquitinated by the anaphase promoting complex (APC), leading to its proteasomal degradation.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in skeletal muscle.

Subunit Structure:

Interacts with DACH1 and EYA3 (By similarity). Interacts with EYA1 and EYA2. Interacts with CDC20. Interacts with TBX18.

Family&Domains:

Belongs to the SIX/Sine oculis homeobox family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). An EMT-related genes signature as a prognostic biomarker for patients with endometrial cancer. BMC Cancer, 2023 (PubMed: 37723477) [IF=3.8]
2). Six1 Promotes Epithelial-Mesenchymal Transition in Bronchial Epithelial Cells via the TGFβ1/Smad Signalling Pathway. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2021 (PubMed: 33631753) [IF=2.8]

Application: WB    Species: Human    Sample: 16HBE cells

Fig. 2. TGFβ1 promotes EMT and increases Six1 in 16HBE cells. A Phenotypic changes were observed through an inverted microscope: normal group of 16HBE cells (a); 16HBE cells treated with TGFβ1 for 24 h (b); 16HBE cells treated with TGFβ1 for 48 h (c); and 16HBE cells treated with TGFβ1 for 72 h (magnification, ×40) (d). B The expression of E-cad and α-SMA mRNA was determined by RT-PCR (*p < 0.05, **p < 0.01). C The expression of E-cad and α-SMA was measured using the Western blot analysis (*p < 0.05, **p < 0.01). D The expression of FN mRNA and protein was measured by RT-PCR and Western blot analysis (**p < 0.01). E The expression of Six1 mRNA and protein was measured by RT-PCR and Western blot analysis (**p < 0.01). Six1, sine oculis homeobox homolog 1; E-cad, E-cadherin; EMT, epithelial-mesenchymal transition; α-SMA, α-smooth muscle actin; mRNA, messenger RNA; FN, fibronectin; TGFβ1, transforming growth factor β1.

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