HOXA5 Antibody - #DF4123

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Product: HOXA5 Antibody
Catalog: DF4123
Description: Rabbit polyclonal antibody to HOXA5
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 30kDa,43kDa; 29kD(Calculated).
Uniprot: P20719
RRID: AB_2836488

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
HOXA5 Antibody detects endogenous levels of total HOXA5.
RRID:
AB_2836488
Cite Format: Affinity Biosciences Cat# DF4123, RRID:AB_2836488.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Homeo box 1C; Homeo box A5; Homeobox A5; Homeobox protein Hox-1C; Homeobox protein Hox-A5; Homeobox protein HOXA5; HOX 1; HOX 1.3; HOX 1C; HOX1; HOX1.3; HOX1C; HOXA5; HXA5_HUMAN; MGC9376; OTTHUMP00000217259;

Immunogens

Immunogen:
Uniprot:

P20719(HXA5_HUMAN) >>Visit HPA database.

Gene(ID):
HOXA5(3202)
Sequence:
MSSYFVNSFCGRYPNGPDYQLHNYGDHSSVSEQFRDSASMHSGRYGYGYNGMDLSVGRSGSGHFGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQSEPSPAPPAQPQIYPWMRKLHISHDNIGGPEGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSMAAAGGAFRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
90
Xenopus
75
Chicken
70
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20719 As Substrate

Site PTM Type Enzyme
Y45 Phosphorylation
Y49 Phosphorylation
S55 Phosphorylation
S59 Phosphorylation
S121 Phosphorylation
S147 Phosphorylation
S156 Phosphorylation
T200 Phosphorylation
Y202 Phosphorylation
T203 Phosphorylation
K212 Ubiquitination
R218 Methylation

Research Backgrounds

Function:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the Antp homeobox family.

References

1). Prenatal PM2.5 exposure impairs spatial learning and memory in male mice offspring: from transcriptional regulation to neuronal morphogenesis. Particle and Fibre Toxicology, 2023 (PubMed: 37081511) [IF=7.2]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5 Transcriptional regulation of neuronal morphological development-associated DEGs in male offspring following prenatal PM2.5 exposure. (a) The numbers of DEGs associated with the morphological development of axons, dendrites, and synapses at different windows, with genes selected based on FDR 
2). HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1. IUBMB LIFE, 2021 (PubMed: 34117711) [IF=3.7]

Application: WB    Species: Mice    Sample: RAW264.7 cells

FIGURE 5 HOXA5 reduced the atherosclerotic lesion formation in carotid arteries. (a, b) Relative mRNA and protein expressions of HOXA5 in carotid arteries with atherosclerosis were determined by QPCR and Western blot when HOXA5 was overexpressed. (c, d) Representative images of carotid arterial sections stained with hematoxylin and eosin (HE) or Oil Red O. Black star: medial layer; black triangle: intimal layer; blue triangle: plaque. & p < .05. n = 6. HOXA5, homeobox A5; QPCR, quantitative real-time reverse transcription PCR

Application: IF/ICC    Species: Mice    Sample: RAW264.7 cells

FIGURE 6 HOXA5 promoted M2 macrophage polarization by the co-regulation of MED1 in carotid arteries. (a) Immunofluorescent images for the colocalization of HOXA5 and CD163. (b, d) Relative mRNA levels of M1 (MARCO and MHCII) and M2 (PPARγ, Arg1, and MRC1) markers were detected by QPCR. (c, e) The levels of IL-6 and IL-10 were determined by ELISA. (f, g) Relative mRNA and protein levels of MED1 were examined using QPCR and Western blot. (h) Immunofluorescent images for the co-localization of MED1 and MAC- 3. & p < .05. n = 6. ELISA, enzyme-linked immunosorbent assay; HOXA5, homeobox A5; MED1, mediator subunit 1; QPCR, quantitative real-time reverse transcription PCR

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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