HLA-H Antibody | Affinity Biosciences

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Western blot analysis of extracts from LOVO cells, using HLAH antibody.

DF4117 at 1/100 staining rat testis tissue by IHC-P.

Product:	HLA-H Antibody
Catalog:	DF4117
Description:	Rabbit polyclonal antibody to HLA-H
Application:	WB IHC IF/ICC
Reactivity:	Human
Mol.Wt.:	40 KD; 41kD(Calculated).
Uniprot:	P01893
RRID:	AB_2836482

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

HLA-H Antibody detects endogenous levels of total HLA-H.

RRID:

AB_2836482
Cite Format: Affinity Biosciences Cat# DF4117, RRID:AB_2836482.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HLA-12.4; HLA-AR; HLA-H; HLAH; HLAHP; Major histocompatibility complex, class I, H (pseudogene); MHC class I antigen H; Putative HLA class I histocompatibility antigen, alpha chain H;

Immunogens

Immunogen:

Uniprot:

P01893(HLAH_HUMAN) >>Visit HPA database.

Sequence:

P01893 HLAH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVLMAPRTLLLLLSGALALTQTWARSHSMRYFYTTMSRPGAGEPRFISVGYVDDTQFVRFDSDDASPREEPRAPWMEREGPKYWDRNTQICKAQAQTERENLRIALRYYNQSEGGSHTMQVMYGCDVGPDGPFLRGYEQHAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAARRAEQRRVYLEGEFVEWLRRYLENGKETLQRADPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTVPIVGIVAGLVLLVAVVTGAVVAAVMWRKKSSDRKGGSYSQAASSNSAQGSDVSLTA

PTMs - P01893 As Substrate

P01893

Site	PTM Type	Source
K168	Ubiquitination	Uniprot
K170	Ubiquitination	Uniprot
K200	Ubiquitination	Uniprot
K267	Ubiquitination	Uniprot
S350	Phosphorylation	Uniprot

Research Backgrounds

P01893_HLAH_HUMAN

Function:

Involved in the presentation of foreign antigens to the immune system.

Subcellular Location:

Cell membrane>Single-pass membrane protein.

Subunit Structure:

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).

Family&Domains:

Belongs to the MHC class I family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

HLA-H Antibody - #DF4117