GBP3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from COLO cells using GBP3 antibody.

Product:	GBP3 Antibody
Catalog:	DF4114
Description:	Rabbit polyclonal antibody to GBP3
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	64 KD; 68kD(Calculated).
Uniprot:	Q9H0R5
RRID:	AB_2836479

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

GBP3 Antibody detects endogenous levels of total GBP3.

RRID:

AB_2836479
Cite Format: Affinity Biosciences Cat# DF4114, RRID:AB_2836479.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp686E0974; DKFZp686L15228; FLJ10961; GBP 3; GBP-3; GBP3; GBP3_HUMAN; GTP binding protein 3; GTP-binding protein 3; Guanine nucleotide binding protein 3; Guanine nucleotide-binding protein 3; Guanylate binding protein 3; Guanylate-binding protein 3; guanylate-binding protein 3 delta C; SPG3A;

Immunogens

Immunogen:

Uniprot:

Q9H0R5(GBP3_HUMAN) >>Visit HPA database.

Gene(ID):

GBP3(2635)

Sequence:

Q9H0R5 GBP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAVLLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLEYSLKLTQGTSQKDKNFNLPRLCIRKFFPKKKCFVFDLPIHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIKVNGPRLESLVLTYINAISRGDLPCMENAVLALAQIENSAAVQKAIAHYDQQMGQKVQLPAETLQELLDLHRVSEREATEVYMKNSFKDVDHLFQKKLAAQLDKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYCLFIQKLQDLEKKYYEEPRKGIQAEEILQTYLKSKESVTDAILQTDQILTEKEKEIEVECVKAESAQASAKMVEEMQIKYQQMMEEKEKSYQEHVKQLTEKMERERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQNEIQKLQKTLKKKTKRYMSHKLKI

PTMs - Q9H0R5 As Substrate

Q9H0R5

Site	PTM Type	Source
S52	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
S156	Phosphorylation	Uniprot
S157	Phosphorylation	Uniprot
K276	Ubiquitination	Uniprot
Y298	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot
K451	Ubiquitination	Uniprot
Y462	Phosphorylation	Uniprot
K518	Acetylation	Uniprot
K520	Acetylation	Uniprot
T547	Phosphorylation	Uniprot
S550	Phosphorylation	Uniprot
K551	Ubiquitination	Uniprot

Research Backgrounds

Q9H0R5_GBP3_HUMAN

Function:

Exhibits antiviral activity against influenza virus.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Golgi apparatus membrane.
Note: Heterodimers with GBP1, GBP2 and GBP5 localize in the compartment of the prenylated GBPs: with GBP1 in a vesicle-like compartment, with GBP2, around the nucleus and with GBP-5, at the Golgi apparatus.

Subunit Structure:

Heterodimer with other family members, including GBP1, GBP2 and GBP5. Dimerization regulates subcellular location.

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

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GBP3 Antibody - #DF4114