GDF7 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HT-29 cells, using GDF7 antibody.

Western blot analysis of extracts from HT-29 cells, using GDF7 Antibody.

Product:	GDF7 Antibody
Catalog:	DF4097
Description:	Rabbit polyclonal antibody to GDF7
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Rabbit
Mol.Wt.:	47 KD; 47kD(Calculated).
Uniprot:	Q7Z4P5
RRID:	AB_2836471

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(82%), Bovine(91%), Rabbit(82%)

Clonality:

Polyclonal

Specificity:

GDF7 Antibody detects endogenous levels of total GDF7.

RRID:

AB_2836471
Cite Format: Affinity Biosciences Cat# DF4097, RRID:AB_2836471.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bmp12; bone morphogenetic protein 12; GDF-7; Gdf7; GDF7_HUMAN; growth differentiation factor 7; Growth/differentiation factor 7;

Immunogens

Immunogen:

Uniprot:

Q7Z4P5(GDF7_HUMAN) >>Visit HPA database.

Gene(ID):

GDF7(151449)

Sequence:

Q7Z4P5 GDF7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLSAAAALCLWLLSACRPRDGLEAAAVLRAAGAGPVRSPGGGGGGGGGGRTLAQAAGAAAVPAAAVPRARAARRAAGSGFRNGSVVPHHFMMSLYRSLAGRAPAGAAAVSASGHGRADTITGFTDQATQDESAAETGQSFLFDVSSLNDADEVVGAELRVLRRGSPESGPGSWTSPPLLLLSTCPGAARAPRLLYSRAAEPLVGQRWEAFDVADAMRRHRREPRPPRAFCLLLRAVAGPVPSPLALRRLGFGWPGGGGSAAEERAVLVVSSRTQRKESLFREIRAQARALGAALASEPLPDPGTGTASPRAVIGGRRRRRTALAGTRTAQGSGGGAGRGHGRRGRSRCSRKPLHVDFKELGWDDWIIAPLDYEAYHCEGLCDFPLRSHLEPTNHAIIQTLLNSMAPDAAPASCCVPARLSPISILYIDAANNVVYKQYEDMVVEACGCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Pig

Rabbit

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z4P5 As Substrate

Q7Z4P5

Site	PTM Type	Source
S85	Phosphorylation	Uniprot
Y96	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
S146	Phosphorylation	Uniprot
S147	Phosphorylation	Uniprot
S243	Phosphorylation	Uniprot

Research Backgrounds

Q7Z4P5_GDF7_HUMAN

Function:

May play an active role in the motor area of the primate neocortex.

Subcellular Location:

Secreted.

Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GDF7 Antibody - #DF4097