GDF15 Antibody - #DF4096

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Product: GDF15 Antibody
Catalog: DF4096
Description: Rabbit polyclonal antibody to GDF15
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 37 KD; 34kD(Calculated).
Uniprot: Q99988
RRID: AB_2836470

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(90%), Dog(88%)
Clonality:
Polyclonal
Specificity:
GDF15 Antibody detects endogenous levels of total GDF15.
RRID:
AB_2836470
Cite Format: Affinity Biosciences Cat# DF4096, RRID:AB_2836470.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GDF 15; GDF-15; Gdf15; GDF15_HUMAN; Growth differentiation factor 15; Growth/differentiation factor 15; Macrophage inhibitory cytokine 1; MIC 1; Mic-1; MIC1; NAG 1; NAG-1; NAG1; NRG 1; NRG-1; NRG1; NSAID (nonsteroidal anti inflammatory drug) activated protein 1; NSAID; NSAID regulated protein 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; PDF; PLAB; Placental bone morphogenetic protein; Placental bone morphogenic protein; Placental TGF beta; Placental TGF-beta; Prostate differentiation factor; PTGF beta; PTGFB;

Immunogens

Immunogen:
Uniprot:

Q99988(GDF15_HUMAN) >>Visit HPA database.

Gene(ID):
GDF15(9518)
Expression:
Q99988 GDF15_HUMAN:

Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney (PubMed:9348093). Detected in plasma (at protein level) (PubMed:28572090, PubMed:29046435).

Sequence:
MPGQELRTVNGSQMLLVLLVLSWLPHGGALSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQLELHLRPQAARGRRRARARNGDHCPLGPGRCCRLHTVRASLEDLGWADWVLSPREVQVTMCIGACPSQFRAANMHAQIKTSLHRLKPDTVPAPCCVPASYNPMVLIQKTDTGVSLQTYDDLLAKDCHCI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
90
Dog
88
Xenopus
67
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99988 As Substrate

Site PTM Type Enzyme
S44 O-Glycosylation
S146 Phosphorylation
K265 Ubiquitination
K303 Ubiquitination

Research Backgrounds

Function:

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling (By similarity).

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Detected in plasma (at protein level).

Subunit Structure:

Homodimer; disulfide-linked. Interacts with GFRAL; ligand of GFRAL which mediates GDF15 internalization and cellular signaling through interaction with RET.

Family&Domains:

Belongs to the TGF-beta family.

References

1). Qizhen capsule inhibits colorectal cancer by inducing NAG-1/GDF15 expression that mediated via MAPK/ERK activation. Journal of Ethnopharmacology, 2021 (PubMed: 33640439) [IF=5.4]

Application: WB    Species: mice    Sample: tumor tissue

Fig. 8. QZC significantly reduces HCT116 xenograft tumor growth in nude mice. Mice were divided into model, QZCL (1.5 g/kg), QZCH (4.5 g/kg), QZCL/5-FU (1.5 g/kg+5-FU), and 5-FU groups (n = 12 mice/group). (A) Rate of tumor formation. (B) Xenograft tumor volumes (mm3 ) in nude mice. (C) Average final tumor weight. (D) Images of all tumors from all five groups. (E) Body weight change in nude mice. (F) Expression of the PARP, FoxO3a, AMPK and MAPK/ERK proteins in xenograft tumors, as determined using Western blot analyses. (G–I) The expression levels of NAG-1 in tumors and serum from nude mice, as determined using Western blotting (G), qRT-PCR (H), and ELISA (I). The relative intensities of all proteins were calculated after normalization to the total protein or β-actin. Data are presented as the means ± SE. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the model group; ##p < 0.05 for the comparison between the QZCH and model groups; △△p < 0.01 for the comparison between the 5-FU and model groups; and ◆◆p < 0.01 for the comparison between the QZCL/5-FU and model groups.
2). A label-free amperometric immunosensor with improved electrocatalytic 3D braided AuPtCu-SWCNTs@MoS2-rGO for human growth differentiation factor-15 detection. Analytical Methods, 2022 (PubMed: 35315459) [IF=3.1]

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